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Impact of matrix pr...
Impact of matrix properties on survival of freeze-dried bacteria
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- Wessman, Per (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Mircobiology, SLU, Swedish University of Agricultural Sciences
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- Mahlin, Denny (författare)
- Uppsala universitet,Institutionen för farmaci
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- Akhtar, Sultan (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Rubino, Stefano, 1978- (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Leifer, Klaus, 1965- (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Kessler, Vadim (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kemi,Department of Chemistry,Department of Chemistry, SLU, Swedish University of Agricultural Sciences
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- Håkansson, Sebastian (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Microbiology, SLU, Swedish University of Agricultural Sciences
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(creator_code:org_t)
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- 2011-03-28
- 2011
- Engelska.
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Ingår i: Journal of the Science of Food and Agriculture. - : Wiley. - 0022-5142 .- 1097-0010. ; 91:14, s. 2518-2528
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Background: Disaccharides are in general first choice as formulation compounds when freezedrying microorganisms. Although polysaccharides and other biopolymers are considered too large to stabilize and interact with cell components in the same beneficial way as disaccharides, polymers have been reported to support cell survival. In the present study we compare the efficiency of sucrose, the polymers Ficoll, hydroxyethylcellulose, hydroxypropylmethylcellulose and polyvinylalcohol to support survival of three bacterial strains during freeze-drying. The initial osmotic conditions were adjusted to be similar for all formulations. Formulation characterization was used to interpret the impact that different compound properties had on cell survival. Results: Despite differences in molecular size, both sucrose and the sucrose based polymer Ficoll supported cell survival after freeze-drying equally well. All formulations became amorphous upon dehydration. Scanning electron microscopy and X-ray diffraction data showed that the discerned differences in structure of the dry formulations had little impact on the survival rates. The capability of the polymers to support cell survival correlated with the surface activity of the polymers in a similar way for all investigated bacterial strains. Conclusion: Polymer-based formulations can support cell survival as effectively as disaccharides if formulation properties of importance for maintaining cell viability are identified and controlled.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Nyckelord
- formulation
- freeze-drying
- biopolymer
- sucrose
- bacteria
- surface
- Food science
- Livsmedelsvetenskap
- TECHNOLOGY
- TEKNIKVETENSKAP
- Biologi med inriktning mot mikrobiologi
- Biology with specialization in Microbiology
- Teknisk fysik med inriktning mot materialvetenskap
- Engineering Science with specialization in Materials Science
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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