Search: id:"swepub:oai:DiVA.org:uu-147259" >
Femtosecond diffrac...
Femtosecond diffractive imaging of biological cells
-
- Seibert, M. Marvin (author)
- Uppsala universitet,Molekylär biofysik
-
- Boutet, Sebastien (author)
- Uppsala universitet,Molekylär biofysik
-
- Svenda, Martin (author)
- Uppsala universitet,Molekylär biofysik
-
show more...
-
- Ekeberg, Tomas (author)
- Uppsala universitet,Molekylär biofysik
-
- Maia, Filipe R. N. C. (author)
- Uppsala universitet,Molekylär biofysik
-
Bogan, Michael J. (author)
-
- Timneanu, Nicusor (author)
- Uppsala universitet,Molekylär biofysik
-
Barty, Anton (author)
-
Hau-Riege, Stefan (author)
-
- Caleman, Carl (author)
- Uppsala universitet,Molekylär biofysik
-
Frank, Matthias (author)
-
Benner, Henry (author)
-
Lee, Joanna Y. (author)
-
Marchesini, Stefano (author)
-
Shaevitz, Joshua W. (author)
-
Fletcher, Daniel A. (author)
-
Bajt, Sasa (author)
-
- Andersson, Inger (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Molekylär biofysik,Institutionen för molekylärbiologi,Department of Molecular Biology
-
Chapman, Henry N. (author)
-
- Hajdu, Janos (author)
- Uppsala universitet,Molekylär biofysik
-
show less...
-
(creator_code:org_t)
-
- 2010-09-17
- 2010
- English.
-
In: Journal of Physics B. - : IOP Publishing. - 0953-4075 .- 1361-6455. ; 43:19, s. 194015-
- Related links:
-
https://hal.archives...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://res.slu.se/i...
-
show less...
Abstract
Subject headings
Close
- In a flash diffraction experiment, a short and extremely intense x-ray pulse illuminates the sample to obtain a diffraction pattern before the onset of significant radiation damage. The over-sampled diffraction pattern permits phase retrieval by iterative phasing methods. Flash diffractive imaging was first demonstrated on an inorganic test object (Chapman et al 2006 Nat. Phys. 2 839-43). We report here experiments on biological systems where individual cells were imaged, using single, 10-15 fs soft x-ray pulses at 13.5 nm wavelength from the FLASH free-electron laser in Hamburg. Simulations show that the pulse heated the sample to about 160 000 K but not before an interpretable diffraction pattern could be obtained. The reconstructed projection images return the structures of the intact cells. The simulations suggest that the average displacement of ions and atoms in the hottest surface layers remained below 3 angstrom during the pulse.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Seibert, M. Marv ...
-
Boutet, Sebastie ...
-
Svenda, Martin
-
Ekeberg, Tomas
-
Maia, Filipe R. ...
-
Bogan, Michael J ...
-
show more...
-
Timneanu, Nicuso ...
-
Barty, Anton
-
Hau-Riege, Stefa ...
-
Caleman, Carl
-
Frank, Matthias
-
Benner, Henry
-
Lee, Joanna Y.
-
Marchesini, Stef ...
-
Shaevitz, Joshua ...
-
Fletcher, Daniel ...
-
Bajt, Sasa
-
Andersson, Inger
-
Chapman, Henry N ...
-
Hajdu, Janos
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Atom and Molecul ...
- Articles in the publication
-
Journal of Physi ...
- By the university
-
Uppsala University
-
Swedish University of Agricultural Sciences