Sökning: id:"swepub:oai:DiVA.org:uu-148608" >
Structural Basis of...
Structural Basis of Oligosaccharide Receptor Recognition by Human Papillomavirus
-
Dasgupta, Jhimli (författare)
-
Bienkowska-Haba, Malgorzata (författare)
-
Ortega, Marcos E. (författare)
-
visa fler...
-
Patel, Hetalkumar D. (författare)
-
- Bodevin, Sabrina (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Spillmann, Dorothe (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
Bishop, Brooke (författare)
-
Sapp, Martin (författare)
-
Chen, Xiaojiang S. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2011
- 2011
- Engelska.
-
Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 286:4, s. 2617-2624
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- High risk human papillomavirus types 16 (HPV16) and 18 (HPV18) can cause cervical cancer. Efficient infection by HPV16 and HPV18 pseudovirions requires interactions of particles with cell-surface receptor heparan sulfate oligosaccharide. To understand the virus-receptor interactions for HPV infection, we determined the crystal structures of HPV16 and HPV18 capsids bound to the oligosaccharide receptor fragment using oligomeric heparin. The HPV-heparin structures revealed multiple binding sites for the highly negatively charged oligosaccharide fragment on the capsid surface, which is different from previously reported virus-receptor interactions in which a single type of binding pocket is present for a particular receptor. We performed structure-guided mutagenesis to generate mutant viruses, and cell binding and infectivity assays demonstrated the functional role of viral residues involved in heparin binding. These results provide a basis for understanding virus-heparan sulfate receptor interactions critical for HPV infection and for the potential development of inhibitors against HPV infection.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas