Search: id:"swepub:oai:DiVA.org:uu-149561" >
The Instant Blood-M...
The Instant Blood-Mediated Inflammatory Reaction Characterized in Hepatocyte Transplantation
-
- Gustafson, Elisabet K. (author)
- Uppsala universitet,Barnkirurgi
-
- Elgue, Graciela (author)
- Uppsala universitet,Klinisk immunologi
-
Hughes, Robin D. (author)
-
show more...
-
Mitry, Ragai R. (author)
-
- Sanchez, Javier (author)
- Uppsala universitet,Klinisk immunologi
-
- Haglund, Ulf (author)
- Uppsala universitet,Gastrointestinalkirurgi
-
- Meurling, Staffan (author)
- Uppsala universitet,Barnkirurgi
-
Dhawan, Anil (author)
-
- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
-
- Nilsson, Bo (author)
- Uppsala universitet,Klinisk immunologi,Forskargrupp Bo Nilsson
-
show less...
-
(creator_code:org_t)
- 2011
- 2011
- English.
-
In: Transplantation. - 0041-1337 .- 1534-6080. ; 91:6, s. 632-638
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background. Hepatocyte transplantation (HcTx) has proven to be a safe procedure, although the functional results have been unsatisfactory, probably due to insufficient engraftment or a loss of transplanted mass or function. In this study, we investigate whether hepatocytes in contact with blood induce an inflammatory reaction leading to, similar to what happens in clinical islet transplantation, an instant blood-mediated inflammatory reaction (IBMIR) resulting in an early loss of transplanted cells. Methods. By using an experimental model that mimics the portal vein blood flow, we could study different parameters reflecting the effects on the innate immunity elicited by hepatocytes in contact with ABO-matched human blood. Results. We report that all aspects of the IBMIR such as platelet and granulocyte consumption, coagulation, and complement activation were demonstrated. Addition of various specific inhibitors of coagulation allowed us to clearly delineate the various stages of the hepatocyte-triggered IBMIR and show that the reaction was triggered by tissue factor. Analysis of a case of clinical HcTx showed that hepatocyte-induced IBMIR also occurs in vivo. Both the inflammatory and the coagulation aspects were controlled by low-molecular-weight dextran sulfate. Conclusion. Isolated hepatocytes in contact with blood induce the IBMIR in vitro, and there are indications that these events are also relevant in vivo. According to these findings, HcTx would benefit from controlling a wider range of signals from the innate immune system.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- IBMIR
- Islet transplantation
- Cell transplantation
- Innate immunity
- Engraftment
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Gustafson, Elisa ...
-
Elgue, Graciela
-
Hughes, Robin D.
-
Mitry, Ragai R.
-
Sanchez, Javier
-
Haglund, Ulf
-
show more...
-
Meurling, Staffa ...
-
Dhawan, Anil
-
Korsgren, Olle
-
Nilsson, Bo
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Surgery
- Articles in the publication
-
Transplantation
- By the university
-
Uppsala University