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Simultaneous visual...
Simultaneous visualization of both signaling cascade activity and end-point gene expression in single cells
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- Weibrecht, Irene (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Molecular Medicine
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- Grundberg, Ida, 1982- (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Molekylär Diagnostik
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- Nilsson, Mats (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
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- Söderberg, Ola (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Molecular Medicine
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(creator_code:org_t)
- 2011-05-25
- 2011
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:5, s. e20148-
- Relaterad länk:
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have developed an approach for simultaneous detection of individual endogenous protein modifications and mRNA molecules in single cells in situ. For this purpose we combined two methods previously developed in our lab: in situ proximity ligation assay for the detection of individual protein interactions and -modifications and in situ detection of single mRNA molecules using padlock probes. As proof-of-principle, we demonstrated the utility of the method for simultaneous detection of phosphorylated PDGFRβ and DUSP6/MKP-3 mRNA molecules in individual human fibroblasts upon PDGF-BB stimulation. Further we applied drugs disrupting the PDGFRβ signaling pathway at various sites to show that this combined method can concurrently monitor the molecular effect of the drugs, i.e. inhibition of downstream signaling from the targeted node in the signaling pathway. Due to its ability to detect different types of molecules in single cells in situ the method presented here can contribute to a deeper understanding of cell-to-cell variations and can be applied to e.g. pinpoint effector sites of drugs in a signaling pathway.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- in situ PLA
- padlock probe
- rolling circle amplification
- single molecule
- single cell
- Molecular medicine (genetics and pathology)
- Molekylär medicin (genetik och patologi)
- Molekylär medicin
- Molecular Medicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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