Application of a fast multi element screening method using ICP-AES on liver samples and mice organs

• A fast mix and measure method for direct multi element analysis of non digested biological samples was used for the simultaneous determination of low, medium, and high concentrations elements in liver samples from domestic and wild animals. The method was also used for screening several elements in different organs from five mice. Brain (cerebrum, left and right cerebellum), heart, kidney, liver, lung, spleen, and blood samples were analysed. The total time for sample preparation and measurement is four minutes only for each sample (dissection not included). Quantitative results within ± 10 % are presented in the liver samples for Al, Cd, Pb, Mn, Sr, P, K, and semi quantitative within ± 20 % for Ca, Co, Cu, Fe, Mg, and Zn. The overall reproducibility was around 5 % for most elements, slightly higher for elements close to the detection limit, which meets well with the 2-3 % obtained for the acid digestion method used for comparison. A bovine liver sample was spiked with low amounts of Al, Cd, Co, and Pb to outline the possibilities of the slurry method to quantify concentrations close to the estimated limit of quantification. The recoveries were 103, 104, 97 and 103 % respectively.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

multi element

screening

medical samples

biological samples

ICP-AES

inductively coupled plasma atomic emission spectrometry

liver

mice

Analytical chemistry

Analytisk kemi

Analytical Chemistry

Analytisk kemi

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