T Cells Stimulated With an Analog Peptide of Type II Collagen Require the Fc Receptor gamma-Chain to Secrete Interleukin-4 and Suppress Autoimmune Arthritis in Mice

• Objective. To explore the characteristics of the T cell population that responds to an analog peptide (A9) of type II collagen and regulates autoimmunity, using the collagen-induced arthritis (CIA) model. Methods. Analog peptide A9 is a 26-amino acid peptide analogous to the sequence of a segment of type II collagen (CII(245-270)) but with substitutions at amino acid positions 260 (alanine for isoleucine), 261 (hydroxyproline for alanine), and 263 (asparagine for phenylalanine). We previously showed that A9 profoundly suppressed CIA and immune responses to type II collagen. In order to determine the mechanism of suppression, we used transgenic mice whose T cells express a type II collagen-specific receptor (T cell receptor) and performed passive cell transfer experiments. Results. The results demonstrated that suppression of CIA by A9 is dependent on T cells. Using multiparameter flow cytometry, we determined that the cells responsible for suppression were CD4+ and expressed high levels of Fc epsilon receptor I gamma chain (FcR gamma). To establish the significance of this finding, we obtained mice genetically deficient in FcR gamma in order to perform passive transfer experiments. The resulting FcR gamma(-/-) CD4+ T cells, when primed by culture with A9, could not transfer the suppression of arthritis or secrete cytokines in response to A9. Conclusion. Taken together, the results of this study suggest that the suppression of arthritis and the Th2 cytokine profile elicited by A9 is dependent on the presence of FcR gamma in T cells. These findings are novel and may have therapeutic potential for patients with autoimmune arthritis.

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