Yeast surviving factor Svf1 as a new interacting partner, regulator and in vitro substrate of protein kinase CK2

• Since Svf1 is phosphoprotein, we investigated whether it was a substrate for protein kinase CK2. According to the amino acid sequence Svf1 harbours 20 putative CK2 phosphorylation sites. Here, we have reported cloning, overexpression, purification and characterization of yeast Svf1 as a substrate for three forms of yeast CK2. Svf1 serves as a substrate for both the recombinant CK2alpha (K (m) 0.35 muM) and CK2alpha' (K (m) 0.18 muM) as well as CK2 holoenzyme (K (m) 1.1 muM). Different K (m) values argue that CK2beta(beta') subunit has an inhibitory effect on the activity of both CK2alpha and CK2alpha' towards surviving factor Svf1. Reconstitution of alpha'(2)betabeta' isoform of CK2 holoenzyme shows that beta/beta' subunits have regulatory effect depending on the kind of CK2 catalytic subunit. This effect was not observed in the case of alpha(2)betabeta' isoform, which may be due to interaction between Svf1 and regulatory CK2beta subunit (shown by co-immunoprecipitation experiments). Interactions between CK2 subunits and Svf1 protein may have influence on ATP as well as ATP-competitive inhibitors (TBBt and TBBz) binding. CK2 phosphorylates up to six serine residues in highly acidic peptide K(199)EVIPESDEEESSADEDDNEDEDEESGDSEEESGSEEESDSEEVEITYED(248) of the Svf1 protein in vitro. Presented data may help to elucidate the role of protein kinase CK2 and Svf1 in the regulation of cell survival pathways.

Nyckelord

Yeast

Protein kinase CK2

Phosphorylation

Apoptosis

Svf1

Mass spectrometry

Svf1–CK2b interaction

ATP-competitive inhibitors

MEDICINE

MEDICIN

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