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Induction of Gliobl...
Induction of Glioblastoma Multiforme and Gliomatosis Cerebri with a Sleeping Beauty gene transfer system, implications for T regulatory cell involvement during glioma formation.
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- Caglayan, Demet (författare)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Moriarity, Branden (författare)
- Department of Genetics, Cell biology and Development, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
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- Tschida, Barbara Ryan (författare)
- Department of Genetics, Cell biology and Development, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
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- Kastemar, Marianne (författare)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Alafuzoff, Irina (författare)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Largaespada, David (författare)
- Department of Genetics, Cell biology and Development, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
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- Westermark, Bengt (författare)
- Uppsala universitet,Cancer och vaskulärbiologi
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Glioblastoma Multiforme (GBM), the most malignant and common neoplasm of the central nervous system (CNS), has been classified into subgroups with gene-expression profile as the basis for categorization. Among these the mesenchymal subgroup is most greatly associated with inflammatory infiltrates and increased expression of inflammatory associated genes. GBMs exhibit T cell infiltration to a varying degree and today the degree of infiltration is not used in prognostics. The Sleeping Beauty (SB) system was used to introduce AKT, a mutant variant of NRAS and a shp53 coupled to green fluorescent protein (GFP) into mice that are fully immunocomptetent, lack mature T cells or have reduced regulatory T (Treg) cell function respectively. We report, for the first time, the induction of Gliomatosis Cerebri with the SB system. Tumors that originated were either GBM or Gliomatosis Cerebri with a similar incidence. There was no difference in survival, grade or incidence of induced tumors in wild type mice and mice that lack mature T cells.
Nyckelord
- : brain tumors
- Sleeping Beauty
- T cells
- AKT
- NRAS
- shp53
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)