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Keratinocytes and H...
Keratinocytes and Head and Neck Squamous Cell Carcinoma Cells Regulate Urokinase-type Plasminogen Activator and Plasminogen Activator Inhibitor-1 in Fibroblasts
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- Hakelius, Malin (författare)
- Uppsala universitet,Plastikkirurgi,Dept Surg Sci, Uppsala University, Uppsala, Sweden
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- Koskela, Anita (författare)
- Region Örebro län,Clin Res Ctr
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- Ivarsson, Mikael (författare)
- Clin Res Ctr, Örebro University Hosp, Örebro, Sweden
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- Grenman, Reidar (författare)
- Dept Otorhinolaryngol Head & Neck Surg & Med Bio, Turku University Hosp, Turku, Finland; University Turku, Turku, Finland
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- Rubin, Kristofer (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Dept Med Biochem & Microbiol, Uppsala University, Uppsala, Sweden
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- Gerdin, Bengt (författare)
- Uppsala universitet,Plastikkirurgi,Dept Surg Sci, Uppsala University, Uppsala, Sweden
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- Nowinski, Daniel (författare)
- Uppsala universitet,Plastikkirurgi,Dept Surg Sci, Uppsala University, Uppsala, Sweden
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(creator_code:org_t)
- 2013
- 2013
- Engelska.
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 33:8, s. 3113-3118
- Relaterad länk:
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: To investigate possible differences in the effects of soluble factors from oral squamous cell carcinoma (SCC) cells (UT-SCC-87) and normal oral keratinocytes (NOK) on fibroblast expression of genes involved in tumor stroma turnover. Materials and Methods: Transwell co-cultures with fibroblasts in collagen gels, and SCC cells or NOK in inserts were carried out. Fibroblast gene expression was measured with real-time polymerase chain reaction (PCR). Results: The expression of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) was up-regulated in co-cultures with SCC cells but not with NOK. In contrast, both SCC cells and NOK regulated matrix metalloproteinase-1 (MMP1) and -3, and tissue inhibitor of metalloproteinases-2 (TIMP2) and -3 to a similar extent, while MMP2 and TIMP1 were largely unaffected. Interleukin 1 alpha (IL1 alpha) up-regulated both MMP1 and MMP3 and down-regulated PAI-1, TIMP2 and -3. Conclusion: SCC and NOK regulate fibroblast expression of genes involved in tumor stroma turnover differentially in vitro. These observations may contribute to a better understanding of the mechanisms behind extracellular matrix turnover in tumors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Head and neck cancer
- keratinocytes
- differential regulation
- PAI-1
- uPA
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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