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The cGMP-inhibitabl...
The cGMP-inhibitable phosphodiesterase modulates glucose transport activation by insulin
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- Eriksson, Jan W (författare)
- Department of Medicine, University of Göteborg, Sahlgren's Hospital, Sweden
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Wesslau, C (författare)
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Smith, U (författare)
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1189:2, s. 163-167
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- To assess the role of the cGMP-inhibitable phosphodiesterase (cGI-PDE) in the action of insulin on glucose transport, adipocytes from young, lean rats were preincubated for 20 min at 37 degrees C with and without OPC 3911, a specific inhibitor of cGI-PDE, and 3-O-methylglucose uptake was measured. Insulin-stimulated glucose transport was impaired by OPC 3911 (approximately 15%) and this impairment became more pronounced in the presence of the degradable cAMP-analogue 8-bromo-cAMP (approximately 45%). This analogue alone did not significantly decrease glucose transport. Furthermore, insulin sensitivity was impaired by the combination of OPC 3911 and 8-bromo-cAMP. Maximal insulin-stimulated glucose transport in adipocytes from aging, obese rats was affected similarly by OPC 3911 and 8-bromo-cAMP, suggesting that cGI-PDE activity is not markedly altered in this insulin-resistant state. In conclusion, cGI-PDE exerts a modulating effect on the stimulatory action of insulin on glucose transport. This effect is particularly pronounced when the cellular cAMP levels are elevated.
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