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Co-culture of insul...
Co-culture of insulin producing human EndoC-βH1 cells with boundary cap neural crest stem cells protects partially against cytokine-induced cell death
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- Ngamjariyawat, Anongnad, 1976- (författare)
- Uppsala universitet,Institutionen för neurovetenskap,Regenerative Neurobiology
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- Turpaev, Kyril (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Vasylovska, Svitlana (författare)
- Uppsala universitet,Institutionen för neurovetenskap,Regenerative Neurobiology
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- Welsh, Nils (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Kozlova, Elena (författare)
- Regenerative Neurobiology
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- Fred, Rikard (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We have recently observed that co-culture of mouse and rat beta-cells with mouse boundary cap neural crest stem cells (bNCSCs) protected against inflammatory cytokine-induced cell death, possibly via direct cadherin-mediated cell-to-cell junctions. However, it has not been addressed whether also human beta-cells can be protected via this strategy. The aim of this investigation was therefore to study the effect of bNCSC co-culture with insulin producing human EndoC-βH1 cells on cytokine-induced cell death. For this purpose GFP-positive bNCSCs were cultured together with GFP-negative EndoC-βH1 cells in the presence of the cytokines IL-1b (50 U/ml) and IFN-g (1000 U/ml). Cells were then stained with propidium iodide and trypsinized for flow cytometry analysis. Analysis of propidium iodide fluorescence in GFP-positive and GFP-negative cells revealed that EndoC-βH1 cells died to a lower extent when co-cultured with bNCSCs than when cultured without bNCSCs. We also observed that EndoC-βH1 cells formed N-cadherin, but not E-cadherin junctions with the bNCSCs. The bNCSC cell population contained a large proportion of beta-tubulin expressing cells indicating neuronal differentiation. A protective function of the N-cadherin junctions was suggested by one experiment in which a neutralizing N-cadherin antibody counteracted the effect of co-culture. We conclude that the interaction between human insulin producing cells and bNCSCs results in a lowered susceptibility of insulin producing cells to pro-inflammatory cytokines in vitro.
Nyckelord
- Biomedical Laboratory Science
- Biomedicinsk laboratorievetenskap
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)