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Loop-mediated isoth...
Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar
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Cook, Jackie (författare)
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- Aydin-Schmidt, Berit (författare)
- Karolinska Institutet
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Gonzalez, Iveth J. (författare)
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visa fler...
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Bell, David (författare)
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- Edlund, Elin (författare)
- Karolinska Institutet
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Nassor, Majda H. (författare)
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Msellem, Mwinyi (författare)
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- Ali, Abdullah (författare)
- Karolinska Institutet
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Abass, Ali K. (författare)
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- Mårtensson, Andreas (författare)
- Uppsala universitet,Centrum för klinisk forskning i Sörmland (CKFD)
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Bjorkman, Anders (författare)
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visa färre...
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(creator_code:org_t)
- 2015-01-28
- 2015
- Engelska.
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Ingår i: Malaria Journal. - : Springer Science and Business Media LLC. - 1475-2875 .- 1475-2875. ; 14, s. 43-
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://malariajourn...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
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- Background: Asymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput detection of asymptomatic malaria infections during mass screening and treatment (MSAT) in Zanzibar, a malaria pre-elimination setting. Methods: Screening took place in three known hotspot areas prior to the short rains in November. Finger-prick blood was taken for screening by rapid diagnostic test (RDT) and LAMP and collected on filter paper for subsequent polymerase chain reaction (PCR) analyses. LAMP results were compared to RDT and to PCR using McNemar's test. Results: Approximately 1,000 people were screened. RDT detected ten infections (1.0% (95% CI 0.3-1.6)) whilst both LAMP and PCR detected 18 (1.8% (95% CI 0.9-2.6)) infections. However, PCR identified three infections that LAMP did not detect and vice versa. LAMP testing was easy to scale-up in field conditions requiring minimal training and equipment, with results ready one to three hours after screening. Conclusions: Despite lower than expected prevalence, LAMP detected a higher number of infections than the currently used diagnostic, RDT. LAMP is a field-friendly, sensitive diagnostic test that could be useful for MSAT malaria campaigns which require quick results to enable prompt treatment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Nyckelord
- LAMP
- Low-density
- Malaria
- Zanzibar
- Elimination
- Asymptomatic
- Diagnostics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Cook, Jackie
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Aydin-Schmidt, B ...
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Gonzalez, Iveth ...
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Bell, David
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Edlund, Elin
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Nassor, Majda H.
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visa fler...
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Msellem, Mwinyi
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Ali, Abdullah
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Abass, Ali K.
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Mårtensson, Andr ...
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Bjorkman, Anders
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Annan klinisk me ...
- Artiklar i publikationen
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Malaria Journal
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet