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A host factor suppo...
A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein
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- Schmith, Anika (författare)
- Univ Jena, Inst Pharm, Dept Pharmaceut Biol, D-07743 Jena, Germany.
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- Spaller, Thomas (författare)
- Univ Jena, Inst Pharm, Dept Pharmaceut Biol, D-07743 Jena, Germany.
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- Gaube, Friedemann (författare)
- Univ Jena, Inst Pharm, Dept Pharmaceut Biol, D-07743 Jena, Germany.
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- Fransson, Åsa (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology,Swedish Univ Agr Sci, Biomed Ctr, Dept Mol Biol, Uppsala, Sweden.
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- Boesler, Benjamin (författare)
- Univ Kassel, Inst Biol Genet, D-34125 Kassel, Germany.
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- Ojha, Sandeep (författare)
- Jacobs Univ Bremen, Ribogenet Biochem Lab, Dept Life Sci & Chem, Mol Life Sci Res Ctr, D-28759 Bremen, Germany.
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- Nellen, Wolfgang (författare)
- Univ Kassel, Inst Biol Genet, D-34125 Kassel, Germany.
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- Hammann, Christian (författare)
- Jacobs Univ Bremen, Ribogenet Biochem Lab, Dept Life Sci & Chem, Mol Life Sci Res Ctr, D-28759 Bremen, Germany.
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- Söderbom, Fredrik (författare)
- Uppsala universitet,Mikrobiologi
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- Winckler, Thomas (författare)
- Univ Jena, Inst Pharm, Dept Pharmaceut Biol, D-07743 Jena, Germany.
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Univ Jena, Inst Pharm, Dept Pharmaceut Biol, D-07743 Jena, Germany Institutionen för molekylärbiologi (creator_code:org_t)
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- 2015-09-03
- 2015
- Engelska.
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Ingår i: Mobile DNA. - : Springer Science and Business Media LLC. - 1759-8753. ; 6
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https://uu.diva-port... (primary) (Raw object)
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https://mobilednajou...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: In the compact and haploid genome of Dictyostelium discoideum control of transposon activity is of particular importance to maintain viability. The non-long terminal repeat retrotransposon TRE5-A amplifies continuously in D. discoideum cells even though it produces considerable amounts of minus-strand (antisense) RNA in the presence of an active RNA interference machinery. Removal of the host-encoded C-module-binding factor (CbfA) from D. discoideum cells resulted in a more than 90 % reduction of both plus-and minus-strand RNA of TRE5-A and a strong decrease of the retrotransposition activity of the cellular TRE5-A population. Transcriptome analysis revealed an approximately 230-fold overexpression of the gene coding for the Argonaute-like protein AgnC in a CbfA-depleted mutant. Results: The D. discoideum genome contains orthologs of RNA-dependent RNA polymerases, Dicer-like proteins, and Argonaute proteins that are supposed to represent RNA interference pathways. We analyzed available mutants in these genes for altered expression of TRE5-A. We found that the retrotransposon was overexpressed in mutants lacking the Argonaute proteins AgnC and AgnE. Because the agnC gene is barely expressed in wild-type cells, probably due to repression by CbfA, we employed a new method of promoter-swapping to overexpress agnC in a CbfA-independent manner. In these strains we established an in vivo retrotransposition assay that determines the retrotransposition frequency of the cellular TRE5-A population. We observed that both the TRE5-A steady-state RNA level and retrotransposition rate dropped to less than 10 % of wild-type in the agnC overexpressor strains. Conclusions: The data suggest that TRE5-A amplification is controlled by a distinct pathway of the Dictyostelium RNA interference machinery that does not require RNA-dependent RNA polymerases but involves AgnC. This control is at least partially overcome by the activity of CbfA, a factor derived from the retrotransposon's host. This unusual regulation of mobile element activity most likely had a profound effect on genome evolution in D. discoideum.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- Dictyostelium
- Retrotransposition
- siRNA
- RNAi
- Argonaute
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Schmith, Anika
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Spaller, Thomas
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Gaube, Friedeman ...
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Fransson, Åsa
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Boesler, Benjami ...
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Ojha, Sandeep
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Nellen, Wolfgang
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Hammann, Christi ...
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Söderbom, Fredri ...
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Winckler, Thomas
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Genetik
- Artiklar i publikationen
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Mobile DNA
- Av lärosätet
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Uppsala universitet
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Sveriges Lantbruksuniversitet