Studies on the active site of deacetoxycephalosporin C synthase

• The Fe(II) and 2-oxogolutarate-dependent dioxygenase deacetoxycephalosporin C synthase (DAOCS) from Streptomyces clavuligerus was expressed at ca 25% of total soluble protein in Escherichia coli and purified by an efficient large-scale procedure. Purified

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

antibiotic biosynthesis; dioxygenase; EXAFS; iron; 2-oxoglutarate; ISOPENICILLIN-N-SYNTHASE; CURVED-WAVE THEORY; X-RAY-ABSORPTION; RING EXPANSION; STREPTOMYCES-CLAVULIGERUS; PENICILLIN BIOSYNTHESIS; EXAFS CALCULATIONS; ESCHERICHIA-COLI; CEPHALOSPORIN; ENZ

Biochemistry

Biokemi

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