Sökning: id:"swepub:oai:DiVA.org:uu-284058" >
Spinal Cord Interne...
Spinal Cord Interneurons Expressing the Gastrin-Releasing Peptide Receptor Convey Itch Through VGLUT2-Mediated Signaling
-
- Aresh, Bejan, 1984- (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
- Freitag, Fabio B. (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
- Perry, Sharn (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Funktionella neuronala nätverk
-
visa fler...
-
- Blümel, Edda (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
- Lau, Joey, 1979- (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Forskargrupp Per-Ola Carlsson
-
- Franck, Marina C.M. (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensoriska kretsar
-
- Lagerström, Malin C. (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
visa färre...
-
(creator_code:org_t)
- 2017-02-01
- 2017
- Engelska.
-
Ingår i: Pain. - : Ovid Technologies (Wolters Kluwer Health). - 0304-3959 .- 1872-6623. ; 158:5, s. 945-961
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://europepmc.or...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Itch is a sensation that promotes the desire to scratch, which can be evoked by mechanical and chemical stimuli. In the spinal cord, neurons expressing the gastrin-releasing peptide receptor (GRPR) have been identified as specific mediators of itch. However, our understanding of the GRPR population in the spinal cord, and thus how these neurons exercise their functions, is limited. For this purpose, we constructed a Cre line designed to target the GRPR population of neurons (Grpr-Cre). Our analysis revealed that Grpr-Cre cells in the spinal cord are predominantly excitatory interneurons that are found in the dorsal lamina, especially in laminae II-IV. Application of the specific agonist gastrin-releasing peptide induced spike responses in 43.3% of the patched Grpr-Cre neurons, where the majority of the cells displayed a tonic firing property. Additionally, our analysis showed that the Grpr-Cre population expresses Vglut2 mRNA, and mice ablated of Vglut2 in Grpr-Cre cells (Vglut2-lox; Grpr-Cre mice) displayed less spontaneous itch and attenuated responses to both histaminergic and nonhistaminergic agents. We could also show that application of the itch-inducing peptide, natriuretic polypeptide B, induces calcium influx in a subpopulation of Grpr-Cre neurons. To summarize, our data indicate that the Grpr-Cre spinal cord neural population is composed of interneurons that use VGLUT2-mediated signaling for transmitting chemical and spontaneous itch stimuli to the next, currently unknown, neurons in the labeled line of itch.
Nyckelord
- Itch
- Gastrin-releasing peptide receptor population
- Natriuretic polypeptide B
- Spinal cord
- Vesicular glutamate transporter 2
- Neuronal networks
- Labeled line of itch
- Electrophysiology
- Conditional knockout analysis
- Tracing
- Calcium imaging
- Grpr
- VGLUT2
- Medicinsk vetenskap
- Medical Science
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
Pain
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas