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Duplex detection of...
Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene
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- Abdeldaim, Guma (författare)
- Uppsala universitet,Klinisk bakteriologi,Benghazi Univ, Fac Med, Dept Med Microbiol & Parasitol, Benghazi, Libya.
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- Svensson, Erik (författare)
- Statens Serum Inst, Int Reference Lab Mycobacteriol, Copenhagen, Denmark.
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- Blomberg, Jonas (författare)
- Uppsala universitet,Klinisk virologi
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- Herrmann, Björn (författare)
- Uppsala universitet,Klinisk bakteriologi
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(creator_code:org_t)
- 2016-09-28
- 2016
- Engelska.
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Ingår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley. - 0903-4641 .- 1600-0463. ; 124:11, s. 991-995
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other nonrespiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non-tuberculous mycobacteria detected by culture. Six of them were detected by the PCR assay.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Mycobacterium tuberculosis
- non-tuberculosis mycobacteria
- real-time PCR
- rnpB
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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