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Nanometer resolutio...
Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
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- Balzarotti, Francisco (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.
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- Eilers, Yvan (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.
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- Gwosch, Klaus C. (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.
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- Gynnå, Arvid H. (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi
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- Westphal, Volker (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.
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- Stefani, Fernando D. (författare)
- Consejo Nacl Invest Cient & Tecn, Ctr Invest Bionanociencias CIBION, Buenos Aires, DF, Argentina.;Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Fis, Buenos Aires, DF, Argentina.
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- Elf, Johan (författare)
- Uppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab
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- Hell, Stefan W. (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Max Planck Inst Med Res, Dept Opt Nanoscopy, Heidelberg, Germany.;German Canc Res Ctr, Opt Nanoscopy Div, Heidelberg, Germany.
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Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany Science for Life Laboratory, SciLifeLab (creator_code:org_t)
- 2016-12-22
- 2017
- Engelska.
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 355:6325, s. 606-612
- Relaterad länk:
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http://ri.conicet.go...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolutionmicroscopy, MINFLUXattained similar to 1-nanometer precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli. As conceptual limits have not been reached, we expect this localization modality to break new ground for observing the dynamics, distribution, and structure of macromolecules in living cells and beyond.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
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