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Lactose repressor-o...
Lactose repressor-operator DNA interactions : kinetic analysis by a surface plasmon resonance biosensor.
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- Bondeson, K (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Frostell-Karlsson, A (författare)
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Fägerstam, L (författare)
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visa fler...
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Magnusson, G (författare)
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visa färre...
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(creator_code:org_t)
- 1993
- 1993
- Engelska.
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Ingår i: Analytical Biochemistry. - 0003-2697 .- 1096-0309. ; 214:1, s. 245-51
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Lactose repressor binding to operator DNA and subsequent dissociation of the complex was monitored continuously by a biosensor, measuring surface plasmon resonance. In this analysis a synthetic, double-stranded oligonucleotide containing the operator site was immobilized on the sensor surface and repressor protein was passed over the surface. The formation of the repressor-operator complex was specific and could be inhibited by isopropyl-beta-D-thiogalactopyranoside inducer. From the association curve, the apparent kass was determined to be 1.8 x 10(6) M-1 s-1. Dissociation of the complex was, for the first time for the lac repressor, determined as an uncatalyzed reaction and the kdiss was determined to be 3.4 x 10(-4) s-1. As a reference, the repressor-operator interaction was analyzed by electrophoretic mobility shift assay under similar reaction conditions. With this method the equilibrium binding constant was calculated to be 2.4 (+/- 0.2) x 10(8) M-1. The corresponding value calculated from biosensor data was 5.1 x 10(9) M-1.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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