SwePub
Sök i LIBRIS databas

  Utökad sökning

id:"swepub:oai:DiVA.org:uu-320482"
 

Sökning: id:"swepub:oai:DiVA.org:uu-320482" > Looking for Missing...

Looking for Missing Proteins in the Proteome of Human Spermatozoa : An Update

Vandenbrouck, Yves (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Lane, Lydie (författare)
Univ Geneva, Dept Human Prot Sci, Fac Med, 1 Rue Michel Servet, CH-1211 Geneva 4, Switzerland.;CMU, SIB, CALIPHO Grp, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland.
Carapito, Christine (författare)
Univ Strasbourg, CNRS UMR7178, Lab Spectrometrie Masse BioOrgan LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France.
visa fler...
Duek, Paula (författare)
CMU, SIB, CALIPHO Grp, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland.
Rondel, Karine (författare)
Inserm U1085, Irset, Protim, Campus Beaulieu, F-35042 Rennes, France.
Bruley, Christophe (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Macron, Charlotte (författare)
Univ Strasbourg, CNRS UMR7178, Lab Spectrometrie Masse BioOrgan LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France.
de Peredo, Anne Gonzalez (författare)
Univ Toulouse, CNRS, UPS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France.
Coute, Yohann (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Chaoui, Karima (författare)
Univ Toulouse, CNRS, UPS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France.
Com, Emmanuelle (författare)
Inserm U1085, Irset, Protim, Campus Beaulieu, F-35042 Rennes, France.
Gateau, Alain (författare)
CMU, SIB, CALIPHO Grp, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland.
Hesse, Anne-Marie (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Marcellin, Marlene (författare)
Univ Toulouse, CNRS, UPS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France.
Mear, Loren (författare)
Inserm U1085, Irset, Protim, Campus Beaulieu, F-35042 Rennes, France.
Mouton-Barbosa, Emmanuelle (författare)
Univ Toulouse, CNRS, UPS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France.
Robin, Thibault (författare)
Ctr Univ Informat, Proteome Informat Grp, Route Drize 7, CH-1227 Carouge, Switzerland.
Burlet-Schiltz, Odile (författare)
Univ Toulouse, CNRS, UPS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France.
Cianferani, Sarah (författare)
Univ Strasbourg, CNRS UMR7178, Lab Spectrometrie Masse BioOrgan LSMBO, 25 Rue Becquerel, F-67087 Strasbourg, France.
Ferro, Myriam (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Freour, Thomas (författare)
CHU Nantes, Serv Med Reprod, 38 Blvd Jean Monnet, F-44093 Nantes, France.;INSERM UMR1064, F-44093 Nantes, France. Uppsala Univ, Sci Life Lab, Dept Immunol Genet & Pathol, S-75185 Uppsala, Sweden.
Lindskog, Cecilia (författare)
Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
Garin, Jerome (författare)
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France.;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France.
Pineau, Charles (författare)
Inserm U1085, Irset, Protim, Campus Beaulieu, F-35042 Rennes, France.
visa färre...
CEA, DRF, BIG, Lab Biol Grande Echelle, 17 Rue Martyrs, F-38054 Grenoble, France;Inserm U1038, 17 Rue Martyrs, F-38054 Grenoble, France.;Univ Grenoble, F-38054 Grenoble, France. Univ Geneva, Dept Human Prot Sci, Fac Med, 1 Rue Michel Servet, CH-1211 Geneva 4, Switzerland.;CMU, SIB, CALIPHO Grp, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland. (creator_code:org_t)
2016-08-23
2016
Engelska.
Ingår i: Journal of Proteome Research. - : AMER CHEMICAL SOC. - 1535-3893 .- 1535-3907. ; 15:11, s. 3998-4019
Relaterad länk:
https://hal.archives...
visa fler...
https://urn.kb.se/re...
https://doi.org/10.1...
visa färre...
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • The Chromosome-Centric Human Proteome Project (C-HPP) aims to identify "missing" proteins in the neXtProt knowledgebase. We present an in-depth proteomics analysis of the human sperm proteome to identify testis-enriched missing proteins. Using protein extraction procedures and LC-MS/MS analysis, we detected 235 proteins (PE2-PE4) for which no previous evidence of protein expression was annotated. Through LC-MS/MS and LC-PRM analysis, data mining, and immunohistochemistry, we confirmed the expression of 206 missing proteins (PE2-PE4) in line with current HPP guidelines (version 2.0). Parallel reaction monitoring acquisition and sythetic heavy labeled peptides targeted 36 "one-hit wonder" candidates selected based on prior peptide spectrum match assessment. 24 were validated with additional predicted and specifically targeted peptides. Evidence was found for 16 more missing proteins using immunohistochemistry on human testis sections. The expression pattern for some of these proteins was specific to the testis, and they could possibly be valuable markers with fertility assessment applications. Strong evidence was also found of four "uncertain" proteins (PE5); their status should be re-examined. We show how using a range of sample preparation techniques combined with MS-based analysis, expert knowledge, and complementary antibody-based techniques can produce data of interest to the community. All MS/MS data are available via ProteomeXchange under identifier PXD003947. In addition to contributing to the C-HPP, we hope these data will stimulate continued exploration of the sperm proteome.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

human proteome project
spermatozoon
missing proteins
mass spectrometry proteomics
immunohistochemistry
bioinformatics
data mining
cilia

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy