Coeliac disease : Clinical and immunological aspects

• A better immunological definition of coeliac disease and highly discriminatory serum markers are needed to identify children with early mucosal lesions and for rapid follow-up and a better knowledge of antigen reactivity and cytokine production will be needed to clarify the pathogenic mechanisms.The numbers of circulating IgAanti-gliadin antibody-producing (IgAAGA SFC) cells were increased in patients with untreated coeliac disease compared to controls and treated coeliac disease patients. In children with coeliac disease the numbers of IgAAGA SFC increased rapidly after gluten challenge. The levels of IgA specific for human as well as guinea-pig tissue transglutaminase (tTG) were increased in the untreated coeliac diseasechildren compared to the control groups. A human erythrocyte tTG ELISAassay had the highest sensitivity (100%) and a specificity of 98%.The numbers of IFN-γ producing cells in the peripheral blood was increased in children with untreated celiac disease as compared to healthy controls. The IL-4 production correlated with the serum levels of total IgE. The numbers of IFN-γ producing cells increased after gluten challenge, whereas no such change was evident for IL-4 or IL-10 producing cells. Children with coeliac disease had more mononuclear cells expressing TGF-β1, TNF-αand IFN-γ in the lamina propria as compared to disease controls. TGFβ3 and IL-4 expressing cells were present in the lamina propria as well as in the epithelial layer in children with coeliac disease.In conclusion, our results indicate that: (1) the ELISPOT assay or other methods for detection of antibody production may be helpful in assessing the optimal timing of the biopsy to shorten the duration of gluten challenge, (2) anti-tTG IgA antibodies can be used as a sensitive and specific complement to existing serological tests for coeliac disease, (3) circulating mononuclear cells in children with active coeliac disease secrete cytokines compatible with a type 1 response, (4) mononuclear cells in the gut of children with activecoeliac disease produce type 1 cytokines as well asTGF-β and IL-4.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

Oncology

Coeliac disease

cytokines

TGF-beta

tissue transglutaminase

IL-4

IFN-gamma

TNF-alpha

ELISPOT

anti-endomysiumantibodies

anti-gliadin antibodies

Onkologi

Oncology

Onkologi

Klinisk immunologi

Clinical Immunology

Publikations- och innehållstyp

vet (ämneskategori)

dok (ämneskategori)