Sökning: id:"swepub:oai:DiVA.org:uu-328740" >
Standardizing the f...
Standardizing the freeze-thaw preparation of growth factors from platelet lysate
-
- Strandberg, Gabriel (författare)
- Uppsala universitet,Klinisk immunologi
-
- Sellberg, Felix (författare)
- Uppsala universitet,Klinisk immunologi
-
- Sommar, Pehr (författare)
- Karolinska Inst
-
visa fler...
-
- Ronaghi, Martin (författare)
- Uppsala universitet,Klinisk immunologi
-
- Lubenow, Norbert (författare)
- Uppsala universitet,Klinisk immunologi
-
- Knutson, Folke (författare)
- Uppsala universitet,Klinisk immunologi
-
- Berglund, David, 1984- (författare)
- Uppsala universitet,Klinisk immunologi
-
visa färre...
-
(creator_code:org_t)
- 2017-02-09
- 2017
- Engelska.
-
Ingår i: Transfusion. - : WILEY. - 0041-1132 .- 1537-2995. ; 57:4, s. 1058-1065
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure.STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, and 30 freeze-thaw cycles. The resulting growth factor and cytokine concentrations from PPP, PA, and PL of different cycles were analyzed and compared using enzyme-linked immunosorbent assay and multiplex bead assays.RESULTS: PL produced by the freeze-thaw procedure resulted in approximately four-to 10-fold enrichment of transforming growth factor-b1, epidermal growth factor, PLT-derived growth factor (PDGF)-AB/BB, PLT factor-4, and fibroblast growth factor-2. The increase in concentrations plateaued at Cycles 3 and 5 and in some cases declined with further cycles. The concentrations of insulin-like growth factor-1, hepatocyte growth factor, vascular endothelial growth factor, and bone morphogenetic protein-2 in PL were essentially comparable to those in PPP.CONCLUSION: Using the freeze-thaw method, optimal preparation of PL with regard to the concentration of growth factors was achieved at Cycles 3 to 5. Based on our findings, the clinical significance of using a greater number of cycles is likely limited.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas