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T cell receptor ass...
T cell receptor assessment in autoimmune disease requires access to the most adjacent immunologically active organ
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- Oftedal, Bergithe E. (författare)
- Ctr Canc Biol, Dept Genet & Mol Pathol, Adelaide, SA, Australia.;Univ Bergen, Dept Clin Sci, N-5021 Bergen, Norway.
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- Lundgren, Brita Ardesjö (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Ctr Canc Biol, Dept Genet & Mol Pathol, Adelaide, SA, Australia
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- Hamm, David (författare)
- Adapt Biotechnol, 1551 Eastlake Ave E 200, Seattle, WA 98102 USA.
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- Gan, Poh-Yi (författare)
- Monash Univ, Ctr Inflammatory Dis, Dept Med, Clayton, Vic, Australia.
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- Holdsworth, Stephen R. (författare)
- Monash Univ, Ctr Inflammatory Dis, Dept Med, Clayton, Vic, Australia.
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- Hahn, Christopher N. (författare)
- Ctr Canc Biol, Dept Genet & Mol Pathol, Adelaide, SA, Australia.;Univ Adelaide, Sch Med, Adelaide, SA, Australia.
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- Schreiber, Andreas W. (författare)
- SA Pathol, Ctr Canc Biol, ACRF Canc Genom Facil, Adelaide, SA, Australia.;Univ Adelaide, Sch Med, Adelaide, SA, Australia.
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- Scott, Hamish S. (författare)
- Ctr Canc Biol, Dept Genet & Mol Pathol, Adelaide, SA, Australia.;SA Pathol, Ctr Canc Biol, ACRF Canc Genom Facil, Adelaide, SA, Australia.;Univ South Australia, Div Hlth Sci, Sch Pharm & Med Sci, Adelaide, SA, Australia.;Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia.;Univ Adelaide, Sch Med, Adelaide, SA, Australia.
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Ctr Canc Biol, Dept Genet & Mol Pathol, Adelaide, SA, Australia;Univ Bergen, Dept Clin Sci, N-5021 Bergen, Norway. Institutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2017
- 2017
- Engelska.
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Ingår i: Journal of Autoimmunity. - : ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. - 0896-8411 .- 1095-9157. ; 81, s. 24-33
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Next generation sequencing of T and B cell receptors is emerging as a valuable and effective method to diagnose and monitor hematopoietic malignancies. So far, this approach has not been fully explored in regard to autoimmune diseases. T cells develop in the thymus where they undergo positive and negative selection, and the autoimmune regulator (Aire) is central in the establishment of immunological tolerance. Loss of Aire leads to severe multiorgan autoimmune disease with infiltration of autoreactive T cells in affected organs. Here, we have utilized next generation sequencing technology to investigate the T cell receptor repertoire in autoimmunity induced by immunization of mice with a self-antigen, myeloper-oxidase. By investigating the T cell receptor repertoire in peripheral blood, spleen and lumbar lymph nodes from naive and immunized Aire -/- mice and wild type littermates, changes in the usage of V and J genes were evident. Our results identify TCR clonotypes which could be potential targets for immune therapy. Also, Aire -/- autoimmunity is driven by a variety of autoantigens where the autoimmune response is highly polyclonal, and access to the most adjacent immunologically active tissue is required to identify T cell receptor sequences that are potentially unique to the antigen in Aire-/- immunized mice.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Next generation sequencing
- T cell receptor repertoire
- Autoimmune regulator
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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