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The neuronal and as...
The neuronal and astrocytic protein SLC38A10 transports glutamine, glutamate, and aspartate, suggesting a role in neurotransmission
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- Hellsten, Sofie V. (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Hägglund, Maria, 1982- (författare)
- Uppsala universitet,Funktionell farmakologi
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- Eriksson, Mikaela (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Fredriksson, Robert (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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(creator_code:org_t)
- 2017-04-26
- 2017
- Engelska.
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Ingår i: FEBS Open Bio. - : WILEY. - 2211-5463. ; 7:6, s. 730-746
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- In brain cells, glutamine transporters are vital to monitor and control the levels of glutamate and GABA. There are 11 members of the SLC38 family of amino acid transporters of which eight have been functionally characterized. Here, we report the first histological and functional characterization of the previously orphan member, SLC38A10. We used pairwise global sequence alignments to determine the sequence identity between the SLC38 family members. SLC38A10 was found to share 20-25% transmembrane sequence identity with several family members, and was predicted to have 11 transmembrane helices. SLC38A10 immunostaining was abundant in mouse brain using a custom-made anti-SLC38A10 antibody and colocalization of SLC38A10 immunoreactivity with markers for neurons and astrocytes was detected. Using Xenopus laevis oocytes overexpressing SLC38A10, we show that SLC38A10 mediates bidirectional transport of L-glutamine, L-alanine, L-glutamate, and D-aspartate, and efflux of L-serine. This profile mostly resembles system A members of the SLC38 family. In conclusion, the bidirectional transport of glutamine, glutamate, and aspartate by SLC38A10, and the immunostaining detected in neurons and astrocytes, suggest that SLC38A10 plays a role in pathways involved in neurotransmission.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- glutamate/GABA-glutamine cycle
- glutamine transport
- immunohistochemistry
- SLC38A10
- solute carriers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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