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Structural basis fo...
Structural basis for MrpH-dependent Proteus mirabilis biofilm formation
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- Jiang, Wangshu (författare)
- Uppsala universitet,Strukturbiologi
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- Ubhayasekera, Wimal (författare)
- Uppsala universitet,Strukturbiologi
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- Breed, Michael C (författare)
- University of Michigan Medical School
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visa fler...
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- Serr, Nina (författare)
- University of Michigan Medical School
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- Pearson, Melanie M (författare)
- University of Michigan Medical School
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- Knight, Stefan D (författare)
- Uppsala universitet,Strukturbiologi
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Proteus mirabilis is a Gram-negative uropathogen and the major causative agent in catheter-associated (CAUTI) and complicated UTIs. Mannose resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and are required for biofilm formation and auto-aggregation, as well as for bladder and kidney colonisation. Here, the X-ray structure of the MR/P tip-located MrpH adhesin is reported. The structure has an unusual fold not previously observed, and contains a transition metal centre with Cu2+ or Zn2+ ligated by three conserved histidine residues and a ligand. Using metal complementation biofilm assays and site directed mutagenesis of the three histidines we show that an intact metal binding site occupied by zinc or copper is essential for MR/P-mediated biofilm formation. The studies presented here provide important clues as to the mechanism of MR/P-mediated biofilm formation and will serve as a starting point for identifying the physiological MR/P receptor(s).
Ämnesord
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Nyckelord
- fimbriae; adhesins; biofilm; urinary tract infection; Proteus mirabilis
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)