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Comparison of Decel...
Comparison of Decellularized Cow and Human Bone for Engineering Bone Grafts with Human Induced Pluripotent Stem Cells
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- Sladkova, Martina (författare)
- New York Stem Cell Fdn Res Inst, 619 West 54th St, New York, NY 10019 USA
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- Cheng, Jiayi (författare)
- New York Stem Cell Fdn Res Inst, 619 West 54th St, New York, NY 10019 USA
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- Palmer, Michael (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Chen, Silvia (författare)
- LifeNet Hlth Fdn, Virginia Beach, VA USA
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- Lin, Charles (författare)
- New York Stem Cell Fdn Res Inst, 619 West 54th St, New York, NY 10019 USA
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- Xia, Wei, Associate Professor (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Yu, Yue Eric (författare)
- Columbia Univ, Dept Biomed Engn, New York, NY USA
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- Zhou, Bin (författare)
- Columbia Univ, Dept Biomed Engn, New York, NY USA
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- Engqvist, Håkan, 1972- (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- de Peppo, Giuseppe Maria (författare)
- New York Stem Cell Fdn Res Inst, 619 West 54th St, New York, NY 10019 USA
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2019
- 2019
- Engelska.
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Ingår i: Tissue Engineering. Part A. - : Mary Ann Liebert Inc. - 1937-3341 .- 1937-335X. ; 25:3-4, s. 288-301
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bone engineering makes it possible to grow unlimited amounts of viable tissue products for basic and applied research, and for clinical applications. A common trend in tissue engineering is the use of decellularized tissue matrices as scaffolding materials, which display structural, mechanical, and biological attributes typical of the native tissue. Due to the limited availability and high cost of human samples, decellularized tissue matrices are typically derived from animal sources. It is unclear, however, whether interspecies differences in tissue parameters will influence the quality of tissue grafts that are engineered using human stem cells. In this study, decellularized cow and human bone scaffolds were compared for engineering bone grafts using human induced pluripotent stem cell-derived mesodermal progenitor cells. After seeding, the cell-scaffold constructs were cultured for 5 weeks in osteogenic medium under dynamic conditions in perfusion bioreactors. The architectural and chemical properties of the scaffolds were studied using microscopic, spectroscopic, and thermogravimetric techniques, while cell behavior and formation of mineralized tissue were assessed using a combination of molecular assays, histological methods, and imaging technologies. The results show that while scaffolds derived from cow and human bone differ somewhat in architecture and composition, both equally support cell viability, tissue growth, and formation of a mineralized bone matrix. Taken together, the results suggest that scaffolds derived from cow bone represent a suitable and convenient alternative to engineer human bone grafts for various biomedical applications.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- biomaterial scaffold
- bone engineering
- induced pluripotent stem cells
- mesenchymal stem cells
- osteogenic differentiation
- tissue decellularization
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Sladkova, Martin ...
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Cheng, Jiayi
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Palmer, Michael
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Chen, Silvia
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Lin, Charles
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Xia, Wei, Associ ...
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visa fler...
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Yu, Yue Eric
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Zhou, Bin
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Engqvist, Håkan, ...
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de Peppo, Giusep ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomaterialveten ...
- Artiklar i publikationen
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Tissue Engineeri ...
- Av lärosätet
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Uppsala universitet