Redesign of substrate-selectivity determining modules of glutathione transferase A1-1 installs high catalytic efficiency with toxic alkenal products of lipid peroxidation

• The evolution of proteins for novel functions involves point mutations and recombinations of domains or structural segments. Mimicking this process by rational design in vitro is still a major challenge. The present report demonstrates that the active sit

Nyckelord

hydroxyalkenal; protein redesign; sequential design; S-TRANSFERASES; ACTIVE-SITE; HETEROLOGOUS EXPRESSION; COENZYME SPECIFICITY; EVOLUTION; ACID; DEHYDROGENASE; ENZYME; DETOXICATION; MUTAGENESIS

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