Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo(3) ubiquinol oxidase, in a detergent-polymer aqueous two-phase system containing metal-chelating polymer

• A system has been developed for selective partitioning of membrane proteins. For the first time, an integral membrane protein, cytochrome bo, ubiquinol oxidase from Escherichia coli, has been affinity partitioned in an aqueous two-phase system. The system

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affinity partitioning; detergent-polymer aqueous two-phase system; MICELLAR SOLUTIONS; TRITON X-114; SEPARATION; SURFACTANT; BEHAVIOR; MIXTURES; EXTRACTION; SOLUBILIZATION; CHROMATOGRAPHY; DEHYDROGENASE

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