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Identification of p...
Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB)
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- Zhu, Yanping (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Qi, Xiaoying (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Yu, Cuicui (författare)
- Qing Dao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Anesthesiol, 20 Yudong Rd, Yantai S264009, Shandong, Peoples R China
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- Yu, Shoujun (författare)
- Binzhou Med Univ, Yantai Affiliated Hosp, Dept Ultrasound, 717 Jinfu Rd, Binzhou 264100, Shandong, Peoples R China
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- Zhang, Chao (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Zhang, Yuan (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Liu, Xiuxiu (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Xu, Yuxue (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Yang, Chunhua (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Jiang, Wenguo (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Tian, Geng (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Li, Xuri (författare)
- Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China
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- Bergquist, Jonas (författare)
- Uppsala universitet,Analytisk kemi,Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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- Zhang, Jiandi (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China;Yantai Zestern Biotech Co LTD, 39 Keji Ave Bioasis, Yantai, Peoples R China
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- Wang, Lei (författare)
- Harbin Med Univ, Affiliated Hosp 1, Dept Thorac Surg, 23 Youzheng St, Harbin 150000, Heilongjiang, Peoples R China
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- Mi, Jia (författare)
- Binzhou Med Univ, Precis Med Res Ctr, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China
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(creator_code:org_t)
- 2019-04-05
- 2019
- Engelska.
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Ingår i: Clinical Proteomics. - : BMC. - 1542-6416 .- 1559-0275. ; 16
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://clinicalprot...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Esophageal cancer (EC) is one of the malignant tumors with a poor prognosis. The early stage of EC is asymptomatic, so identification of cancer biomarkers is important for early detection and clinical practice.Methods: In this study, we compared the protein expression profiles in esophageal squamous cell carcinoma (ESCC) tissues and adjacent normal esophageal tissues from five patients through high-resolution label-free mass spectrometry. Through bioinformatics analysis, we found the differentially expressed proteins of ESCC. To perform the rapid identification of biomarkers, we adopted a high-throughput protein identification technique of Quantitative Dot Blot (QDB). Meanwhile, the QDB results were verified by classical immunohistochemistry.Results: In total 2297 proteins were identified, out of which 308 proteins were differentially expressed between ESCC tissues and normal tissues. By bioinformatics analysis, the four up-regulated proteins (PTMA, PAK2, PPP1CA, HMGB2) and the five down-regulated proteins (Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1 and Vinculin) were selected and validated in ESCC by Western Blot. Furthermore, we performed the QDB and IHC analysis in 64 patients and 117 patients, respectively. The PTMA expression was up-regulated gradually along the progression of ESCC, and the PTMA expression ratio between tumor and adjacent normal tissue was significantly increased along with the progression. Therefore, we suggest that PTMA might be a potential candidate biomarker for ESCC.Conclusion: In this study, label-free quantitative proteomics combined with QDB revealed that PTMA expression was up-regulated in ESCC tissues, and PTMA might be a potential candidate for ESCC. Since Western Blot cannot achieve rapid and high-throughput screening of mass spectrometry results, the emergence of QDB meets this demand and provides an effective method for the identification of biomarkers.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Esophageal squamous cell carcinoma (ESCC)
- Label-free quantitative proteomics
- Prothymosin alpha (PTMA)
- Quantitative Dot Blot (QDB)
Publikations- och innehållstyp
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Till lärosätets databas
- Av författaren/redakt...
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Zhu, Yanping
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Qi, Xiaoying
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Yu, Cuicui
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Yu, Shoujun
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Zhang, Chao
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Zhang, Yuan
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visa fler...
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Liu, Xiuxiu
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Xu, Yuxue
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Yang, Chunhua
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Jiang, Wenguo
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Tian, Geng
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Li, Xuri
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Bergquist, Jonas
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Zhang, Jiandi
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Wang, Lei
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Mi, Jia
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Clinical Proteom ...
- Av lärosätet
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Uppsala universitet