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Luminescent CeO2:Eu...
Luminescent CeO2:Eu3+ nanocrystals for robust in situ H2O2 real-time detection in bacterial cell cultures
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- Henning, Dorian F. (författare)
- Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England
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- Merkl, Padryk (författare)
- Karolinska Institutet
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- Yun, Changhun (författare)
- Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden
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- Iovino, Federico (författare)
- Karolinska Institutet
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- Xie, Ling, 1982- (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Mouzourakis, Eleftherios (författare)
- Univ Ioannina, Dept Phys, GR-45110 Ioannina, Greece
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- Moularas, Constantinos (författare)
- Univ Ioannina, Dept Phys, GR-45110 Ioannina, Greece
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- Deligiannakis, Yiannis (författare)
- Univ Ioannina, Dept Phys, GR-45110 Ioannina, Greece
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- Henriques-Normark, Birgitta (författare)
- Karolinska Institutet
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- Leifer, Klaus, 1965- (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Sotiriou, Georgios A. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- ELSEVIER ADVANCED TECHNOLOGY, 2019
- 2019
- Engelska.
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 132, s. 286-293
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Hydrogen peroxide (H2O2) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume H2O2 linked to a specific biomarker. Optical H2O2 detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor H2O2 levels in real-time. Here, we utilize enzyme-mimetic CeO2 nanocrystals that are sensitive to H2O2 and study the effect of H2O2 presence on their electronic and luminescent properties. We produce and dope with Eu3+ these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the H2O2 concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO2:Eu3+ nanocrystals are combined in a single-step with larger, non-responsive Y2O3:Tb3+ nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of H2O2 concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time H2O2 detection in vitro in cell cultures.
Ämnesord
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Nyckelord
- Nanozymes
- Rare-earth doped nanoparticles
- Hydrogen peroxide
- Flame nanoparticle synthesis
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Henning, Dorian ...
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Merkl, Padryk
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Yun, Changhun
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Iovino, Federico
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Xie, Ling, 1982-
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Mouzourakis, Ele ...
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visa fler...
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Moularas, Consta ...
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Deligiannakis, Y ...
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Henriques-Normar ...
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Leifer, Klaus, 1 ...
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Sotiriou, Georgi ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Materialkemi
- Artiklar i publikationen
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Biosensors & bio ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet