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WAKMAR2, a Long Non...
WAKMAR2, a Long Noncoding RNA Downregulated in Human Chronic Wounds, Modulates Keratinocyte Motility and Production of Inflammatory Chemokines
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- Herter, Eva K. (författare)
- Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden
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- Li, Dongqing (författare)
- Karolinska Institutet
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- Toma, Maria A. (författare)
- Karolinska Institutet
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- Vij, Manika (författare)
- Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden
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- Li, Xi (författare)
- Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden
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- Visscher, Dani (författare)
- Karolinska Inst, Dermatol & Venereol Sect, Dept Med Solna, SE-17176 Stockholm, Sweden
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- Wang, Aoxue (författare)
- Dalian Med Univ, Dept Dermatol, Hosp 2, Dalian, Peoples R China
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- Chu, Tongbin (författare)
- Dalian Med Univ, Hosp 2, Dept Wound Regenerat, Dalian, Peoples R China
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- Sommar, Pehr (författare)
- Karolinska Institutet
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- Blomqvist, Lennart (författare)
- Karolinska Inst, Dept Med Huddinge, Stockholm, Sweden
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- Berglund, David, 1984- (författare)
- Uppsala universitet,Klinisk immunologi
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- Stahle, Mona (författare)
- Karolinska Institutet
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- Wikstrom, Jakob D. (författare)
- Karolinska Institutet
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- Landen, Ning Xu (författare)
- Karolinska Institutet
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(creator_code:org_t)
- ELSEVIER SCIENCE INC, 2019
- 2019
- Engelska.
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Ingår i: Journal of Investigative Dermatology. - : ELSEVIER SCIENCE INC. - 0022-202X .- 1523-1747. ; 139:6, s. 1373-1384
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Chronic wounds represent a major and growing health and economic burden worldwide. A better understanding of molecular mechanisms of normal as well as impaired wound healing is needed to develop effective treatment. Herein we studied the potential role of long noncoding RNA LOC100130476 in skin wound repair. LOC100130476 is an RNA polymerase IIeencoded polyadenylated transcript present in both cytoplasm and nucleus. We found that its expression was lower in wound-edge keratinocytes of human chronic wounds compared to normal wounds of healthy donors and intact skin. In cultured keratinocytes, LOC100130476 expression was induced by TGF-beta signaling. By reducing LOC100130476 expression with antisense oligos or activating its transcription with CRISPR/Cas9 Synergistic Activation Mediator system, we showed that LOC100130476 restricted the production of inflammatory chemokines by keratinocytes, while enhancing cell migration. In line with this, knockdown of LOC100130476 impaired re-epithelization of human ex vivo wounds. Based on these results, we named LOC100130476 wound and keratinocyte migration-associated long noncoding RNA 2 (WAKMAR2). Moreover, we identified a molecular network that may mediate the biological function of WAKMAR2 in keratinocytes using microarray. In summary, our data suggest that WAKMAR2 is an important regulator of skin wound healing and its deficiency may contribute to the pathogenesis of chronic wounds.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Herter, Eva K.
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Li, Dongqing
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Toma, Maria A.
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Vij, Manika
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Li, Xi
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Visscher, Dani
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visa fler...
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Wang, Aoxue
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Chu, Tongbin
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Sommar, Pehr
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Blomqvist, Lenna ...
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Berglund, David, ...
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Stahle, Mona
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Wikstrom, Jakob ...
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Landen, Ning Xu
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Dermatologi och ...
- Artiklar i publikationen
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Journal of Inves ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet