Sökning: id:"swepub:oai:DiVA.org:uu-387253" >
Structural and Muta...
Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens
-
- Hampel, Sabrina (författare)
- Albert Ludwigs Univ Freiburg, Inst Pharmazeut Wissensch, Albertstr 25, D-79104 Freiburg, Germany
-
- Steitz, Jan-Patrick (författare)
- Albert Ludwigs Univ Freiburg, Inst Pharmazeut Wissensch, Albertstr 25, D-79104 Freiburg, Germany
-
- Baierl, Anna (författare)
- Forschungszentrum Julich, IBG Biotechnol 1, Wilhelm Johnen Str, D-52425 Julich, Germany
-
visa fler...
-
- Lehwald, Patrizia (författare)
- Albert Ludwigs Univ Freiburg, Inst Pharmazeut Wissensch, Albertstr 25, D-79104 Freiburg, Germany
-
- Wiesli, Luzia (författare)
- Empa Swiss Fed Labs Mat Sci & Technol, Lab Biointerfaces, Lerchenfeldstr 5, CH-9014 St Gallen, Switzerland
-
- Richter, Michael (författare)
- Empa Swiss Fed Labs Mat Sci & Technol, Lab Biointerfaces, Lerchenfeldstr 5, CH-9014 St Gallen, Switzerland;Fraunhofer Inst Interfacial Engn & Biotechnol IGB, Branch BioCat, Schulgasse 11a, D-94315 Straubing, Germany
-
- Fries, Alexander (författare)
- Albert Ludwigs Univ Freiburg, Inst Pharmazeut Wissensch, Albertstr 25, D-79104 Freiburg, Germany
-
- Pohl, Martina (författare)
- Forschungszentrum Julich, IBG Biotechnol 1, Wilhelm Johnen Str, D-52425 Julich, Germany
-
- Schneider, Gunter (författare)
- Karolinska Institutet
-
- Dobritzsch, Doreen, 1972- (författare)
- Uppsala universitet,Biokemi
-
- Mueller, Michael (författare)
- Albert Ludwigs Univ Freiburg, Inst Pharmazeut Wissensch, Albertstr 25, D-79104 Freiburg, Germany
-
visa färre...
-
(creator_code:org_t)
- 2018-10-11
- 2018
- Engelska.
-
Ingår i: ChemBioChem. - : WILEY-V C H VERLAG GMBH. - 1439-4227 .- 1439-7633. ; 19:21, s. 2283-2292
- Relaterad länk:
-
https://www.dora.lib...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- A wide range of thiamine diphosphate (ThDP)-dependent enzymes catalyze the benzoin-type carboligation of pyruvate with aldehydes. A few ThDP-dependent enzymes, such as YerE from Yersinia pseudotuberculosis (YpYerE), are known to accept ketones as acceptor substrates. Catalysis by YpYerE gives access to chiral tertiary alcohols, a group of products difficult to obtain in an enantioenriched form by other means. Hence, knowledge of the three-dimensional structure of the enzyme is crucial to identify structure-activity relationships. However, YpYerE has yet to be crystallized, despite several attempts. Herein, we show that a homologue of YpYerE, namely, PpYerE from Pseudomonas protegens (59 % amino acid identity), displays similar catalytic activity: benzaldehyde and its derivatives as well as ketones are converted into chiral 2-hydroxy ketones by using pyruvate as a donor. To enable comparison of aldehyde- and ketone-accepting enzymes and to guide site-directed mutagenesis studies, PpYerE was crystallized and its structure was determined to a resolution of 1.55 angstrom.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- asymmetric synthesis
- biocatalysis
- biosynthesis
- C-C coupling
- tertiary alcohols
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Hampel, Sabrina
-
Steitz, Jan-Patr ...
-
Baierl, Anna
-
Lehwald, Patrizi ...
-
Wiesli, Luzia
-
Richter, Michael
-
visa fler...
-
Fries, Alexander
-
Pohl, Martina
-
Schneider, Gunte ...
-
Dobritzsch, Dore ...
-
Mueller, Michael
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
ChemBioChem
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet