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Fusion pore regulat...
Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis
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- Gucek, Alenka (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Gandasi, Nikhil R (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Omar-Hmeadi, Muhmmad (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Bakke, Marit (författare)
- Univ Bergen, Dept Biomed, Bergen, Norway
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- Doskeland, Stein O. (författare)
- Univ Bergen, Dept Biomed, Bergen, Norway
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- Tengholm, Anders, 1971- (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Barg, Sebastian, 1969- (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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(creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2019
- 2019
- Engelska.
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Ingår i: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 8
- Relaterad länk:
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https://doi.org/10.7...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.7...
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Abstract
Ämnesord
Stäng
- Regulated exocytosis establishes a narrow fusion pore as initial aqueous connection to the extracellular space, through which small transmitter molecules such as ATP can exit. Co-release of polypeptides and hormones like insulin requires further expansion of the pore. There is evidence that pore expansion is regulated and can fail in diabetes and neurodegenerative disease. Here, we report that the cAMP-sensor Epac2 (Rap-GEF4) controls fusion pore behavior by acutely recruiting two pore-restricting proteins, amisyn and dynamin-1, to the exocytosis site in insulin-secreting beta-cells. cAMP elevation restricts and slows fusion pore expansion and peptide release, but not when Epac2 is inactivated pharmacologically or in Epac2(-/-) (Rapgef4(-/-)) mice. Consistently, overexpression of Epac2 impedes pore expansion. Widely used antidiabetic drugs (GLP-1 receptor agonists and sulfonylureas) activate this pathway and thereby paradoxically restrict hormone release. We conclude that Epac2/cAMP controls fusion pore expansion and thus the balance of hormone and transmitter release during insulin granule exocytosis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
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