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4 '-Guanidinium-mod...
4 '-Guanidinium-modified siRNA : a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading
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- Nawale, Ganesh N. (författare)
- Uppsala universitet,Polymerkemi
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- Bahadorikhalili, Saeed (författare)
- Uppsala universitet,Polymerkemi
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- Sengupta, Pallabi (författare)
- Bose Inst, Dept Biophys, Biomol NMR & Drug Design Lab, P-1-12 CIT Scheme 7M, Kolkata, India
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- Kadekar, Sandeep (författare)
- Uppsala universitet,Polymerkemi
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- Chatterjee, Subhrangsu (författare)
- Bose Inst, Dept Biophys, Biomol NMR & Drug Design Lab, P-1-12 CIT Scheme 7M, Kolkata, India
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- Varghese, Oommen P., 1977- (författare)
- Uppsala universitet,Polymerkemi
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(creator_code:org_t)
- 2019
- 2019
- Engelska.
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Ingår i: Chemical Communications. - : ROYAL SOC CHEMISTRY. - 1359-7345 .- 1364-548X. ; 55:62, s. 9112-9115
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://pubs.rsc.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We designed novel 4 '-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in approximate to 4.9-fold more efficient knockdown than unmodified siRNA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
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