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Isolation and cultu...
Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants
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- Barcelo, Marta (författare)
- IFAPA Ctr Malaga, Cortijo Cruz S-N, Malaga 29140, Spain
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- Wallin, Anita (författare)
- Uppsala universitet,Fysiologisk botanik
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- Medina, Juan J. (författare)
- IFAPA Ctr Torres Tomejfl, Seville 41200, Spain
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- Gil-Ariza, David J. (författare)
- IFAPA Ctr Malaga, Cortijo Cruz S-N, Malaga 29140, Spain
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- Lopez-Casado, Gloria (författare)
- Univ Malaga, Dept Biol Vegetal, Inst Hortofruticultura Subtrop & Mediterranea La, E-29071 Malaga, Spain
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- Juarez, Jose (författare)
- Ctra Moncada Naquera, IVIA, Km 4-5, Valencia 46113, Spain
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- Sanchez-Sevilla, Jose F. (författare)
- IFAPA Ctr Malaga, Cortijo Cruz S-N, Malaga 29140, Spain
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- Lopez-Encina, Carlos (författare)
- Inst Hortofruticultura Subtrop & Mediterranea La, Malaga 29750, Spain
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- Lopez-Aranda, Jose M. (författare)
- IFAPA Ctr Malaga, Cortijo Cruz S-N, Malaga 29140, Spain
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- Mercado, Jose A. (författare)
- Univ Malaga, Dept Biol Vegetal, Inst Hortofruticultura Subtrop & Mediterranea La, E-29071 Malaga, Spain
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- Pliego-Alfaro, Fernando (författare)
- Univ Malaga, Dept Biol Vegetal, Inst Hortofruticultura Subtrop & Mediterranea La, E-29071 Malaga, Spain
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(creator_code:org_t)
- ELSEVIER, 2019
- 2019
- Engelska.
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Ingår i: Scientia Horticulturae. - : ELSEVIER. - 0304-4238 .- 1879-1018. ; 256
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Protoplasts are an useful biotechnological tool for plant improvement In strawberry, very few studies on protoplast technology have been carried out. In this investigation, a procedure for the isolation and culture of strawberry protoplasts, cv. 'Chandler', has been developed. The effect of several factors affecting the successful isolation of protoplasts and formation of microcalli, e.g. explant source, washing procedure, hormonal composition of the culture medium and protoplast density, were evaluated. For shoot regeneration, microcalli derived from isolated protoplasts were transferred to MS medium supplemented with 0.2 mg l(-1) NAA and either 5 mg l(-1) BA or 3 mgl(-1) TDZ, obtaining a similar regeneration rate, 17%, in both media. Twenty-one independent protoclones were transferred to field conditions for agronomic evaluation. Significant alterations in the growth habit, density of foliage, leaf color and leaf morphology were detected in some lines. Fruit yield was significantly reduced in 15 out of the 21 protoclones evaluated due to a reduction in fruit weight and/or the number of fruits. Ploidy level was unaffected in a sample of 6 lines selected at random; however, a study of genetic stability by using 10 EST-SSR markers showed genetic alterations in all the lines analyzed. Despite the high rate of somaclonal variation detected in the protoclones, some of the lines displayed an agronomical behavior similar to control plants, indicating that this protocol could be useful for genetic improvement in this species.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
Nyckelord
- Agronomic evaluation
- Fragaria x ananassa
- Protoplast isolation
- Protoplast regeneration
- Somaclonal variation
- Strawberry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Barcelo, Marta
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Wallin, Anita
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Medina, Juan J.
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Gil-Ariza, David ...
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Lopez-Casado, Gl ...
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Juarez, Jose
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visa fler...
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Sanchez-Sevilla, ...
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Lopez-Encina, Ca ...
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Lopez-Aranda, Jo ...
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Mercado, Jose A.
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Pliego-Alfaro, F ...
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visa färre...
- Om ämnet
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- LANTBRUKSVETENSKAPER
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Uppsala universitet