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Development of a gp...
Development of a gp60-subtyping method for Cryptosporidium felis
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- Rojas-Lopez, Laura (författare)
- Uppsala universitet,Mikrobiologi,Publ Hlth Agcy Sweden, S-17182 Solna, Sweden
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- Elwin, Kristin (författare)
- Singleton Hosp Sgeti, Cryptosporidium Reference Unit, Publ Hlth Wales Microbiol & Hlth Protect, Swansea SA2 8QA, W Glam, Wales;Swansea Univ, Med Sch, Grove Bldg,Singleton Pk, Swansea SA2 8PP, W Glam, Wales
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- Chalmers, Rachel M. (författare)
- Singleton Hosp Sgeti, Cryptosporidium Reference Unit, Publ Hlth Wales Microbiol & Hlth Protect, Swansea SA2 8QA, W Glam, Wales;Swansea Univ, Med Sch, Grove Bldg,Singleton Pk, Swansea SA2 8PP, W Glam, Wales
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- Enemark, Heidi L. (författare)
- Natl Vet Inst, Ullevalsveien 68, N-0454 Oslo, Norway
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- Beser, Jessica (författare)
- Publ Hlth Agcy Sweden, S-17182 Solna, Sweden
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- Troell, Karin (författare)
- Natl Vet Inst, S-75189 Uppsala, Sweden
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(creator_code:org_t)
- 2020-01-23
- 2020
- Engelska.
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Ingår i: Parasites & Vectors. - : BMC. - 1756-3305. ; 13
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://parasitesand...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Background: Feline cryptosporidiosis is an increasing problem, especially in catteries. In humans, close contact with cats could be a potential source of infection although the risk of contracting cryptosporidiosis caused by Cryptosporidium felis is considered to be relatively low. Sequencing of the 60-kDa glycoprotein gene is a commonly used tool for investigation of the genetic diversity and transmission dynamics of Cryptosporidium species. However, until now the sequence of gp60 from C. felis has not been available and genotyping has been limited to less discriminatory markers, such as 18S rRNA, COWP and HSP70.Methods: We have identified the gp60 orthologue within the genome sequence of C. felis, and used the sequence to design a nested PCR for subtyping purposes. A total of 128 clinical isolates of both feline and human origin, were used to evaluate the marker.Results: Sequence analysis revealed large variations between the different samples. The C. felis gp60 lack the characteristic serine-tract found in many other cryptosporidian orthologues, instead it has an insertion of variable length (361-742 nt). Also, two cases of suspected zoonotic transmission of C. felis between cats and humans were successfully confirmed.Conclusions: We have identified the gp60 gene in C. felis and show how this highly variable marker can be used in epidemiological investigations.
Ämnesord
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Clinical Science (hsv//eng)
Nyckelord
- Cryptosporidiosis
- Molecular typing
- Zoonotic transmission
- 60-kDa glycoprotein
- Source tracking
- Genetic variability
- Epidemiological marker
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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