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Transcriptomic and ...
Transcriptomic and Proteomic Analysis of Clear Cell Foci (CCF) in the Human Non-Cirrhotic Liver Identifies Several Differentially Expressed Genes and Proteins with Functions in Cancer Cell Biology and Glycogen Metabolism
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- Metzendorf, Christoph (författare)
- Uppsala universitet,Medicinsk genetik och genomik,Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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- Wineberger, Katharina (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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- Rausch, Jenny (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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- Cigliano, Antonio (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.;Regensburg Univ Klinikum, Inst Pathol, Franz Josef Str Allee 11, D-93053 Regensburg, Germany.
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- Peters, Kristin (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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- Sun, Baodong (författare)
- Duke Univ, Dept Pediat, Div Med Genet, Med Ctr, Durham, NC 27710 USA.
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- Mennerich, Daniela (författare)
- Univ Oulu, Fac Biochem & Mol Med, Oulu 90570, Finland.;Univ Oulu, Bioctr Oulu, Oulu 90570, Finland.
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- Kietzmann, Thomas (författare)
- Univ Oulu, Fac Biochem & Mol Med, Oulu 90570, Finland.;Univ Oulu, Bioctr Oulu, Oulu 90570, Finland.
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- Calvisi, Diego F. (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.;Regensburg Univ Klinikum, Inst Pathol, Franz Josef Str Allee 11, D-93053 Regensburg, Germany.
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- Dombrowski, Frank (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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- Ribback, Silvia (författare)
- Univ Med Greifswald, Inst Pathol, Friedrich Loeffler Str 23e, D-17475 Greifswald, Germany.
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(creator_code:org_t)
- 2020-09-10
- 2020
- Engelska.
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Ingår i: Molecules. - : MDPI AG. - 1431-5157 .- 1420-3049. ; 25:18, s. 4141-
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Clear cell foci (CCF) of the liver are considered to be pre-neoplastic lesions of hepatocellular adenomas and carcinomas. They are hallmarked by glycogen overload and activation of AKT (v-akt murine thymoma viral oncogene homolog)/mTOR (mammalian target of rapamycin)-signaling. Here, we report the transcriptome and proteome of CCF extracted from human liver biopsies by laser capture microdissection. We found 14 genes and 22 proteins differentially expressed in CCF and the majority of these were expressed at lower levels in CCF. Using immunohistochemistry, the reduced expressions of STBD1 (starch-binding domain-containing protein 1), USP28 (ubiquitin-specific peptidase 28), monad/WDR92 (WD repeat domain 92), CYB5B (Cytochrome b5 type B), and HSPE1 (10 kDa heat shock protein, mitochondrial) were validated in CCF in independent specimens. Knockout of Stbd1, the gene coding for Starch-binding domain-containing protein 1, in mice did not have a significant effect on liver glycogen levels, indicating that additional factors are required for glycogen overload in CCF. Usp28 knockout mice did not show changes in glycogen storage in diethylnitrosamine-induced liver carcinoma, demonstrating that CCF are distinct from this type of cancer model, despite the decreased USP28 expression. Moreover, our data indicates that decreased USP28 expression is a novel factor contributing to the pre-neoplastic character of CCF. In summary, our work identifies several novel and unexpected candidates that are differentially expressed in CCF and that have functions in glycogen metabolism and tumorigenesis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- clear cell foci
- liver
- hepatocellular carcinoma
- pre-neoplastic lesions
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Metzendorf, Chri ...
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Wineberger, Kath ...
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Rausch, Jenny
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Cigliano, Antoni ...
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Peters, Kristin
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Sun, Baodong
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Mennerich, Danie ...
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Kietzmann, Thoma ...
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Calvisi, Diego F ...
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Dombrowski, Fran ...
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Ribback, Silvia
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
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Molecules
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Uppsala universitet