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Sperm Methylome Pro...
Sperm Methylome Profiling Can Discern Fertility Levels in the Porcine Biomedical Model
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- Pértille, Fábio (författare)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska fakulteten,Linköping Univ, Dept Phys Chem & Biol, SE-58183 Linköping, Sweden.;Linköping Univ, Fac Med & Hlth Sci, Div Cell Biol, Dept Biomed & Clin Sci, SE-58183 Linköping, Sweden.
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- Alvarez-Rodriguez, Manuel (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Linköping Univ, Dept Biomed & Clin Sci BKV, SE-58185 Linköping, Sweden.
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- Nery da Silva, Arthur (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten,Linköping Univ, Dept Phys Chem & Biol, SE-58183 Linköping, Sweden.;Univ Passo Fundo, Sch Agron & Vet Med, BR-99052900 Passo Fundo, RS, Brazil.;Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Vet Med & Anim Hlth, Ctr Comparat Studies Sustainabil Hlth & Welf,FMVZ, BR-13635900 Pirassununga, SP, Brazil.
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- Barranco Cascales, Isabel (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Linköping Univ, Dept Biomed & Clin Sci BKV, SE-58185 Linköping, Sweden.;Univ Murcia, Dept Med & Anim Surg, Murcia 30100, Spain.;Univ Girona, Inst Food & Agr Technol, Dept Biol, E-17003 Girona, Spain.
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- Roca, Jordi (författare)
- Univ Murcia, Dept Med & Anim Surg, Murcia 30100, Spain.
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- Guerrero-Bosagna, Carlos M. (författare)
- Linköpings universitet,Uppsala universitet,Miljötoxikologi,Linköping Univ, Dept Phys Chem & Biol, SE-58183 Linköping, Sweden.,Biologi,Tekniska fakulteten,Uppsala Univ, Sweden
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- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Linköping Univ, Dept Biomed & Clin Sci BKV, SE-58185 Linköping, Sweden.
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(creator_code:org_t)
- 2021-03-06
- 2021
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 22:5
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- A combined Genotyping By Sequencing (GBS) and methylated DNA immunoprecipitation (MeDIP) protocol was used to identify-in parallel-genetic variation (Genomic-Wide Association Studies (GWAS) and epigenetic differences of Differentially Methylated Regions (DMR) in the genome of spermatozoa from the porcine animal model. Breeding boars with good semen quality (n = 11) and specific and well-documented differences in fertility (farrowing rate, FR) and prolificacy (litter size, LS) (n = 7) in artificial insemination programs, using combined FR and LS, were categorized as High Fertile (HF, n = 4) or Low Fertile (LF, n = 3), and boars with Unknown Fertility (UF, n = 4) were tested for eventual epigenetical similarity with those fertility-proven. We identified 165,944 Single Nucleotide Polymorphisms (SNPs) that explained 14-15% of variance among selection lines. Between HF and LF individuals (n = 7, 4 HF and 3 LF), we identified 169 SNPs with p <= 0.00015, which explained 58% of the variance. For the epigenetic analyses, we considered fertility and period of ejaculate collection (late-summer and mid-autumn). Approximately three times more DMRs were observed in HF than in LF boars across these periods. Interestingly, UF boars were clearly clustered with one of the other HF or LF groups. The highest differences in DMRs between HF and LF experimental groups across the pig genome were located in the chr 3, 9, 13, and 16, with most DMRs being hypermethylated in LF boars. In both HF and LF boars, DMRs were mostly hypermethylated in late-summer compared to mid-autumn. Three overlaps were detected between SNPs (p <= 0.0005, n = 1318) and CpG sites within DMRs. In conclusion, fertility levels in breeding males including FR and LS can be discerned using methylome analyses. The findings in this biomedical animal model ought to be applied besides sire selection for andrological diagnosis of idiopathic sub/infertility.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- spermatozoa
- genotyping by sequencing (GBS)
- methylated DNA immunoprecipitation (MeDIP)
- artificial insemination
- fertility
- prolificacy
- pig
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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