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Heat-treated human ...
Heat-treated human platelet pellet lysate modulates microglia activation, favors wound healing and promotes neuronal differentiation in vitro
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- Nebie, Ouada (författare)
- Taipei Med Univ, Coll Biomed Engn, Grad Inst Biomed Mat & Tissue Engn, 250 Wu Xing St, Taipei 11031, Taiwan.
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- Barro, Lassina (författare)
- Taipei Med Univ, Coll Biomed Engn, Int PhD Program Biomed Engn, Taipei, Taiwan.
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- Wu, Yu-Wen (författare)
- Taipei Med Univ, Coll Biomed Engn, Grad Inst Biomed Mat & Tissue Engn, 250 Wu Xing St, Taipei 11031, Taiwan.
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- Knutson, Folke (författare)
- Uppsala universitet,Klinisk immunologi
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- Buee, Luc (författare)
- Univ Lille, CHU Lille, INSERM, U1172,Lille Neurosci & Cognit, Lille, France.
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- Devos, David (författare)
- Univ Lille, CHU Lille, INSERM, U1172,Lille Neurosci & Cognit, Lille, France.
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- Peng, Chih-Wei (författare)
- Taipei Med Univ, Coll Biomed Engn, Int PhD Program Biomed Engn, Taipei, Taiwan.;Taipei Med Univ, Coll Biomed Engn, Sch Biomed Engn, Taipei, Taiwan.
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- Blum, David (författare)
- Univ Lille, CHU Lille, INSERM, U1172,Lille Neurosci & Cognit, Lille, France.
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- Burnouf, Thierry (författare)
- Taipei Med Univ, Coll Biomed Engn, Grad Inst Biomed Mat & Tissue Engn, 250 Wu Xing St, Taipei 11031, Taiwan.;Taipei Med Univ, Coll Biomed Engn, Int PhD Program Biomed Engn, Taipei, Taiwan.;Taipei Med Univ, Int PhD Program Cell Therapy & Regenerat Med, Taipei, Taiwan.;Taipei Med Univ, PhD Program Mind Brain & Consciousness, Taipei, Taiwan.
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Taipei Med Univ, Coll Biomed Engn, Grad Inst Biomed Mat & Tissue Engn, 250 Wu Xing St, Taipei 11031, Taiwan Taipei Med Univ, Coll Biomed Engn, Int PhD Program Biomed Engn, Taipei, Taiwan. (creator_code:org_t)
- 2020-02-27
- 2021
- Engelska.
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Ingår i: Platelets. - : Taylor & Francis. - 0953-7104 .- 1369-1635. ; 32:2, s. 226-237
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The neurorestorative efficacy of human platelet lysates in neurodegenerative disorders is still under investigation. Platelets prepared from standard and pathogen reduced platelet concentrates were pelletized, washed, concentrated, and subjected to freeze-thawing. The lysate was heated to 56 degrees C for 30 min and characterized. Toxicity was evaluated using SH-SY5Y neuroblastoma, BV-2 microglial, and EA-hy926 endothelial cells. Inflammatory activity was tested by examining tumor necrosis factor (TNF) and cyclooxygenase (COX)-2 expressions by BV-2 microglia with or without stimulation by lipopolysaccharides (LPS). The capacity to stimulate wound healing was evaluated by a scratch assay, and the capacity to differentiate SH-SY5Y into neurons was also examined. Platelet lysates contained a range of neurotrophins. They were not toxic to SH-SY5Y, EA-hy926, or BV-2 cells, did not induce the expression of TNF or COX-2 inflammatory markers by BV-2 microglia, and decreased inflammation after LPS stimulation. They stimulated the wound closure in the scratch assay and induced SH-SY5Y differentiation as revealed by the increased length of neurites as well as beta 3-tubulin and neurofilament staining. These data confirm the therapeutic potential of platelet lysates in the treatment of disorders of the central nervous system and support further evaluation as novel neurorestorative biotherapy in preclinical models.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- HPL
- human platelet lysates
- neurorestoration
- neurotrophins
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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