LCMS-based untargeted metabolomics of guinea pig perilymph using a novel separation method for sequential analysis of hydrophilic and lipophilic compounds: Studying the effect of hydrogen gas on noise-induced hearing loss

• Noise-induced hearing loss (NIHL) is believed to be caused by oxidative stress and accounts for up to a third of all cases of acquired hearing loss worldwide. The use of hydrogen gas as a mitigating treatment for NIHL has been successfully demonstrated in the past. We here present the application of a novel LC/LC-HRMS method for sequential analysis of hydrophilic and lipophilic compounds on an untargeted metabolomics study of the attenuating effect of hydrogen gas on NIHL in guinea pigs. The study was conducted using perilymph taken from the basal turn of the cochlea. Samples were taken in the acute stage, immediately following noise exposure along with follow-up samples after 1 and 2 weeks. Data analysis using volcano plots and random forest discriminant models revealed differences in the concentration of potassium within the scala tympani indicating effects on the potassium recycling system of the inner ear. Additionally, discriminant levels of glycerophosphorylcholine, a common osmolyte and antioxidant were discovered. In addition to these two compounds, four additional unknown metabolites are described. The findings are in line with previous reports indicating that the protective effect of hydrogen gas is sustained for at least 2 weeks.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

metabolomics

nihl

guinea pigs

perilymph

hydrogen gas

treatment

Analytisk farmaceutisk kemi

Analytical Pharmaceutical Chemistry

Publikations- och innehållstyp

vet (ämneskategori)

ovr (ämneskategori)