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Sökning: id:"swepub:oai:DiVA.org:uu-476108" > Observations of pha...

Observations of phase changes in monoolein during high viscous injection

Wells, Daniel J. (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
Berntsen, Peter (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Balaur, Eugeniu (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
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Kewish, Cameron M. (författare)
Australian Nucl Sci & Technol Org, Australian Synchrotron, 800 Blackburn Rd, Clayton, Vic 3168, Australia.;La Trobe Univ, Sch Mol Sci, Dept Chem & Phys, Bundoora, Vic 3086, Australia
Adams, Patrick (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Sellberg, Jonas A., 1985- (författare)
KTH,Biomedicinsk fysik och röntgenfysik,SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
Binns, Jack (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Boutet, Sebastien (författare)
SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
Broomhall, Hayden (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Caleman, Carl (författare)
Uppsala universitet,Institutionen för fysik och astronomi,DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany
Christofferson, Andrew (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Conn, Charlotte E. (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Dahlqvist, Caroline (författare)
KTH,AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
Flueckiger, Leonie (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Roque, Francisco Gian (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Greaves, Tamar L. (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Hejazian, Majid (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Hunter, Mark (författare)
SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
Jazi, Marjan Hadian (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Jönsson, H. Olof (författare)
KTH,AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden.,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
Pathirannahalage, Sachini Kadaoluwa (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia
Kirian, Richard A. (författare)
Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA,La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
Kozlov, Alex (författare)
Univ Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
Kurta, Ruslan P. (författare)
European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany
Marman, Hugh (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Mendez, Derek (författare)
Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA
Morgan, Andrew (författare)
Univ Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
Nugent, Keith (författare)
Australian Natl Univ, Res Sch Phys, Acton, ACT, Australia
Oberthuer, Dominik (författare)
DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany
Quiney, Harry (författare)
Univ Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
Reinhardt, Juliane (författare)
Australian Nucl Sci & Technol Org, Australian Synchrotron, 800 Blackburn Rd, Clayton, Vic 3168, Australia
Saha, Saumitra (författare)
Univ Melbourne, ARC Ctr Excellence Adv Mol Imaging, Parkville, Vic 3010, Australia
Sellberg, Jonas A. (författare)
KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden
Sierra, Raymond (författare)
SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
Wiedorn, Max (författare)
DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany
Abbey, Brian (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia
Martin, Andrew, V (författare)
RMIT Univ, Sch Sci, Melbourne, Vic 3000, Australia.
Darmanin, Connie (författare)
La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia.
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La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia La Trobe Univ, Sch Comp Engn & Math Sci, La Trobe Inst Mol Sci, Dept Math & Phys Sci, Bundoora, Vic 3086, Australia (creator_code:org_t)
International Union Of Crystallography, 2022
2022
Engelska.
Ingår i: Journal of Synchrotron Radiation. - : International Union Of Crystallography. - 0909-0495 .- 1600-5775. ; 29:3, s. 602-614
Relaterad länk:
https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
https://urn.kb.se/re...
https://doi.org/10.1...
https://urn.kb.se/re...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam. Typically, the carrier medium is a lipidic cubic phase (LCP) media, which can also be used to nucleate and grow the crystals. However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not been reported and hence is not yet well understood. The self-assembled structure of the LCP can be affected by pressure, dehydration and temperature changes, all of which occur during continuous flow injection. These changes to the LCP structure may in turn impact the results of X-ray diffraction measurements from membrane protein crystals. To investigate the influence of HVIs on the structure of the LCP we conducted a study of the phase changes in monoolein/water and monoolein/buffer mixtures during continuous flow injection, at both atmospheric pressure and under vacuum. The reservoir pressure in the HVI was tracked to determine if there is any correlation with the phase behaviour of the LCP. The results indicated that, even though the reservoir pressure underwent (at times) significant variation, this did not appear to correlate with observed phase changes in the sample stream or correspond to shifts in the LCP lattice parameter. During vacuum injection, there was a three-way coexistence of the gyroid cubic phase, diamond cubic phase and lamellar phase. During injection at atmospheric pressure, the coexistence of a cubic phase and lamellar phase in the monoolein/water mixtures was also observed. The degree to which the lamellar phase is formed was found to be strongly dependent on the co-flowing gas conditions used to stabilize the LCP stream. A combination of laboratory-based optical polarization microscopy and simulation studies was used to investigate these observations.

Ämnesord

NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

high-viscosity injection
monoolein
lipidic cubic phase
cooling effect
continuous flow

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