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Ammonia Concentrati...
Ammonia Concentration in the Eluent Influences Fragmentation Pattern of Triacylglycerols in Mass Spectrometry Analysis
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- Velasco, Marta (författare)
- CSIC, Delegac Inst Castilla & Leon, Valladolid 47003, Spain.
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- Balgoma, David (författare)
- Uppsala universitet,Analytisk farmaceutisk kemi,Univ Valladolid, CSIC, Inst Biol & Genet Mol IBGM, Unidad Excelencia, Valladolid 47003, Spain.
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- Montero, Olimpio (författare)
- Univ Valladolid, CSIC, Inst Biol & Genet Mol IBGM, Unidad Excelencia, Valladolid 47003, Spain.
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CSIC, Delegac Inst Castilla & Leon, Valladolid 47003, Spain Analytisk farmaceutisk kemi (creator_code:org_t)
- 2022-05-18
- 2022
- Engelska.
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Ingår i: Metabolites. - : MDPI. - 2218-1989 .- 2218-1989. ; 12:5
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Correct assessment of the fatty acyl at the glycerol sn-2 position in triacylglycerol (TAG) analysis by liquid chromatography and mass spectrometry (LC-MS) is challenging. Ammonium hydroxide (NH4OH) is the preferred choice for the solvent additive for the formation of the ammonium adduct ([M + NH4](+)). In this study, the influence of different NH4OH concentrations in the eluents on TAG adduct formation and fragmentation under LC-MS analysis was assessed. Increasing NH4OH concentrations delayed the chromatographic elution time according to a power function. The [M + NH4](+) and [M + ACN + NH4](+) adducts (where ACN means acetonitrile) were formed at all ammonium concentrations assayed. [M + ACN + NH4](+) predominated above 18.26 mM [NH4OH], and the intensity of [M + NH4](+) dropped. TAG fragmentation for fatty acyl release in the MSE was reduced with increasing [M + ACN + NH4](+) adduct, which suggests that ACN stabilizes the adduct in a way that inhibits the rupture of the ester bonds in TAGs. A linear equation (Hsn-I = a x H[M+NH4]+, where sn-I refers to the sn position of the glycerol (I = 1, 2, or 3) and H is the peak height) was deduced to quantify the dehydroxydiacylglycerol fragment intensity in relation to [M + NH4](+) intensity in the full scan. This equation had a slope mean value of 0.369 +/- 0.058 for the sn-1 and sn-3 positions, and of 0.188 +/- 0.007 for the sn-2 position.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- triacylglycerols
- mass spectrometry
- regioisomery
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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