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Early divergence of...
Early divergence of translation initiation and elongation factors
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- Fer, Evrim (författare)
- Department of Bacteriology University of Wisconsin‐Madison Madison Wisconsin USA;Microbiology Doctoral Training Program University of Wisconsin‐Madison Madison Wisconsin USA;NASA Center for Early Life and Evolution University of Wisconsin‐Madison Madison Wisconsin USA
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- McGrath, Kaitlyn M. (författare)
- Department of Bacteriology University of Wisconsin‐Madison Madison Wisconsin USA;NASA Center for Early Life and Evolution University of Wisconsin‐Madison Madison Wisconsin USA;Department of Molecular and Cellular Biology University of Arizona Tucson Arizona USA
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- Guy, Lionel, PhD, Docent, 1980- (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
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- Hockenberry, Adam J. (författare)
- Department of Integrative Biology The University of Texas at Austin Austin Texas USA
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- Kaçar, Betül (författare)
- Department of Bacteriology University of Wisconsin‐Madison Madison Wisconsin USA;NASA Center for Early Life and Evolution University of Wisconsin‐Madison Madison Wisconsin USA
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(creator_code:org_t)
- 2022-08-19
- 2022
- Engelska.
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Ingår i: Protein Science. - : John Wiley & Sons. - 0961-8368 .- 1469-896X. ; 31:9
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Protein translation is a foundational attribute of all living cells. The translation function carried out by the ribosome critically depends on an assortment of protein interaction partners, collectively referred to as the translation machinery. Various studies suggest that the diversification of the translation machinery occurred prior to the last universal common ancestor, yet it is unclear whether the predecessors of the extant translation machinery factors were functionally distinct from their modern counterparts. Here we reconstructed the shared ancestral trajectory and subsequent evolution of essential translation factor GTPases, elongation factor EF-Tu (aEF-1A/eEF-1A), and initiation factor IF2 (aIF5B/eIF5B). Based upon their similar functions and structural homologies, it has been proposed that EF-Tu and IF2 emerged from an ancient common ancestor. We generated the phylogenetic tree of IF2 and EF-Tu proteins and reconstructed ancestral sequences corresponding to the deepest nodes in their shared evolutionary history, including the last common IF2 and EF-Tu ancestor. By identifying the residue and domain substitutions, as well as structural changes along the phylogenetic history, we developed an evolutionary scenario for the origins, divergence and functional refinement of EF-Tu and IF2 proteins. Our analyses suggest that the common ancestor of IF2 and EF-Tu was an IF2-like GTPase protein. Given the central importance of the translation machinery to all cellular life, its earliest evolutionary constraints and trajectories are key to characterizing the universal constraints and capabilities of cellular evolution.
Ämnesord
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Nyckelord
- ancestral sequence reconstruction
- elongation factor
- initiation factor
- LUCA
- translation
- Biologi med inriktning mot molekylär evolution
- Biology with specialization in Molecular Evolution
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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