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Determination of py...
Determination of pyronaridine in whole blood by automated solid-phase extraction and high-performance liquid chromatography
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- Blessborn, Daniel (författare)
- Dalarna University College, Borlange, Sweden. Department of Analytical Chemistry, Uppsala University, Uppsala, Sweden
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- Lindegårdh, Niklas (författare)
- Högskolan Dalarna,Kemiteknik
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- Ericsson, Örjan (författare)
- Unit of Tropical Pharmacology, Division of Clinical Pharmacology and Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Huddinge, Sweden
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- Hellgren, Urban (författare)
- Karolinska Institutet
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- Bergqvist, Yngve (författare)
- Högskolan Dalarna,Kemiteknik
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Dalarna University College, Borlange, Sweden Department of Analytical Chemistry, Uppsala University, Uppsala, Sweden Kemiteknik (creator_code:org_t)
- Ovid Technologies (Wolters Kluwer Health), 2003
- 2003
- Engelska.
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Ingår i: Therapeutic Drug Monitoring. - : Ovid Technologies (Wolters Kluwer Health). - 0163-4356 .- 1536-3694. ; 25:3, s. 264-270
- Relaterad länk:
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https://urn.kb.se/re...
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http://kipublication...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- A new extraction procedure for the analysis of pyronaridine in whole blood is presented. A weak cation exchanger with a carboxylic acid (CBA) sorbent was found to be a suitable solid phase sorbent for the extraction of pyronaridine. High-performance liquid chromatography with UV detection at 278 nm and an electrochemical detector at +0.75 V is used. The electrochemical detector gives higher selectivity than the UV detector. The separation was performed using a C18 reversed phase column with mobile phase of acetonitrile-phosphate buffer (0.01 mol/L, pH 2.5)- sodium perchlorate (1.0 mol/L; 22:77:1, v/v/v). The within-day RSDs were below 5% at all concentration levels between 75 nmol/L and 1500 nmol/L, and the between-day RSDs were below 14% at all concentration levels. The limit of quantification was about 50 nmol/L in 1000 microL whole blood with an RSD of 20% or less on a day-to-day basis. The stability of pyronaridine is increased if the pH is less than 3 in water solutions. In whole blood, the concentration decreases by about 10% for each freeze-thaw cycle performed. At room temperature (about 22 degrees C), pyronaridine concentration in whole blood decreases by about 10% within 12 to 24 hours.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Analytical chemistry
- Analytisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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