Increased H3K4me3 at Promoter Sites of Pluripotency Genes in Female Human Embryonic Stem Cells

• Pluripotency is the core characteristic of stem cells that allows them to differentiate into any adult tissue type. Studies have identified a sex bias in pluripotency of mice and human embryonic stem cells, leading to a shift towards a more naïve pluripotency state in female cells. The molecular mechanism behind this sex difference has been partially traced back in female mouse cells to an increased X chromosome dosage and a subsequent reduction of MAP kinase signaling based on X-linked genes. In human cells the mechanism has not been clearly identified since the shift in pluripotency state exists even in X inactivated cells. We performed a quantitative ChIP-seq, to compared epigenomic landscapes of male and female hiPSC and have identified an increased H3K4me3 signal at promoter sites of essential pluripotency genes (SOX2, NANOG, OCT4) and at promoters of genes of pluripotency-related signaling pathways. Differential H3K4me3 patterns could contribute to the sex-biased shift in pluripotency state of human embryonic stem cells. 

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

H3K4me3

pluripotency

epigenetics

histone modification

sex difference

human embryonic stem cell

Biologi med inriktning mot zoologisk utvecklingsbiologi

Biology with specialization in Animal Development

Publikations- och innehållstyp

pop (ämneskategori)

ovr (ämneskategori)