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Sensitive quantific...
Sensitive quantification of short-chain fatty acids combined with global metabolomics in microbiome cultures
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- Lin, Weifeng (författare)
- Uppsala universitet,Kemisk biologi för biomarkörer,Science for Life Laboratory, SciLifeLab,Uppsala Univ, Dept Chem, Sci Life Lab, BMC, S-75124 Uppsala, Sweden.
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- Garcia, Fabricio Romero (författare)
- Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Norin, Elisabeth Lissa (författare)
- Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Kart, Didem (författare)
- Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Engstrand, Lars (författare)
- Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Du, Juan (författare)
- Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Globisch, Daniel (författare)
- Uppsala universitet,Kemisk biologi för biomarkörer,Science for Life Laboratory, SciLifeLab,Analytisk kemi,Uppsala Univ, Dept Chem, Sci Life Lab, BMC, S-75124 Uppsala, Sweden.;Karolinska Inst, Ctr Translat Microbiome Res CTMR, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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(creator_code:org_t)
- Royal Society of Chemistry, 2023
- 2023
- Engelska.
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Ingår i: Chemical Communications. - : Royal Society of Chemistry. - 1359-7345 .- 1364-548X. ; 59:39, s. 5843-5846
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The microbiome has been identified to have a key role for the physiology of their human host. One of the major impacts is the clearance of bacterial pathogens. We have now developed a chemoselective probe methodology for the absolute quantification of short-chain fatty acids at low nM concentrations, with high reproducibility and spiked isotope labelled internal standards. Immobilization to magnetic beads allows for separation from the matrix and the tagged metabolites upon bioorthogonal cleavage can be analyzed via UHPLC-MS. The major advantage of our sensitive method is the simple combination with global metabolomics analysis as only a small sample volume is required. We have applied this chemical metabolomics strategy for targeted SCFA analysis combined with global metabolomics on gut microbiome co-cultures with Salmonella and investigated the effect of antibiotic treatment.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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