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Evaluation of domes...
Evaluation of domestic animal sperm head morphology via flow cytometric DNA labelling and pulse shape analysis using bull and stallion spermatozoa as model species
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- Nagy, Szabolcs (författare)
- Hungarian Univ Agr & Life Sci, Inst Anim Sci, Keszthely, Hungary.;Hungarian Univ Agr & Life Sci, Inst Anim Sci, Georgikon Campus,Deak F u 16, H-8360 Keszthely, Hungary.
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- Kovacs, Barnabas (författare)
- Hungarian Univ Agr & Life Sci, Inst Anim Sci, Keszthely, Hungary.
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- Johannisson, Anders (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Cancerfarmakologi och beräkningsmedicin,Swedish Univ Agr Sci, Uppsala, Sweden.,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Uppsala University
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Hungarian Univ Agr & Life Sci, Inst Anim Sci, Keszthely, Hungary;Hungarian Univ Agr & Life Sci, Inst Anim Sci, Georgikon Campus,Deak F u 16, H-8360 Keszthely, Hungary. Hungarian Univ Agr & Life Sci, Inst Anim Sci, Keszthely, Hungary. (creator_code:org_t)
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- John Wiley & Sons, 2023
- 2023
- Engelska.
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Ingår i: Reproduction in domestic animals. - : John Wiley & Sons. - 0936-6768 .- 1439-0531. ; 58:11, s. 1569-1575
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Abstract
Ämnesord
Stäng
- The aim of the present study was to test a rapid, robust flow cytometric technique for the detection of sperm head abnormalities of domestic bulls and stallions. The so-called PulSA approach detects the pulse profiles of propidium-iodide labelled spermatozoa. In the first experiment, species-specific threshold values were established on sperm samples that were tested for sperm head abnormalities with a classic visual morphology analysis. In the second experiment, serial mixtures of bull and stallion spermatozoa mimicking different percentages of sperm head abnormalities were analysed. Non-metric multidimensional scaling showed a clear separation between the normal and mixed samples. The PulSA approach may be a useful tool in identifying sub- or infertile breeding males as well as in studying the evolutionary aspects of sperm morphology and morphometry.
Ämnesord
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Clinical Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Annan veterinärmedicin (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Other Veterinary Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Husdjursvetenskap -- Husdjursvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Animal and Dairy Sience -- Animal and Dairy Science. (hsv//eng)
Nyckelord
- abnormalities
- bull
- PulSA
- sperm quality
- stallion
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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