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Mapping and Functio...
Mapping and Functional Dissection of the Rumpless Trait in Piao Chicken Identifies a Causal Loss of Function Mutation in the Novel Gene Rum
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- Guo, Ying (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,China Agr Univ, State Key Lab Anim Biotech Breeding, CN-100193 Beijing, Peoples R China.;China Agr Univ, Natl Engn Lab Anim Breeding, CN-100193 Beijing, Peoples R China.;Yazhouwan Natl Lab, CN-572024 Sanya, Peoples R China.
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- Tian, Jing (författare)
- China Agr Univ, State Key Lab Anim Biotech Breeding, CN-100193 Beijing, Peoples R China.;China Agr Univ, Natl Engn Lab Anim Breeding, CN-100193 Beijing, Peoples R China.;Inner Mongolia Acad Agr & Anim Husb Sci, CN-010031 Hohhot, Peoples R China.
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- Song, Chi (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Han, Wei (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Zhu, Chunhong (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Li, Huifang (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Zhang, Shuangjie (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Chen, Kuanwei (författare)
- Jiangsu Inst Poultry Sci, Natl Chickens Genet Resources, CN-225125 Yangzhou, Jiangsu, Peoples R China.
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- Li, Ning (författare)
- China Agr Univ, State Key Lab Anim Biotech Breeding, CN-100193 Beijing, Peoples R China.;China Agr Univ, Natl Engn Lab Anim Breeding, CN-100193 Beijing, Peoples R China.
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- Carlborg, Örjan (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Hu, Xiaoxiang (författare)
- China Agr Univ, State Key Lab Anim Biotech Breeding, CN-100193 Beijing, Peoples R China.;China Agr Univ, Natl Engn Lab Anim Breeding, CN-100193 Beijing, Peoples R China.
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(creator_code:org_t)
- Oxford University Press, 2023
- 2023
- Engelska.
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Ingår i: Molecular biology and evolution. - : Oxford University Press. - 0737-4038 .- 1537-1719. ; 40:12
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Rumpless chickens exhibit an abnormality in their tail development. The genetics and biology of this trait has been studied for decades to illustrate a broad variation in both the types of inheritance and the severity in the developmental defects of the tail. In this study, we created a backcross pedigree by intercrossing Piao (rumpless) with Xianju (normal) to investigate the genetic mechanisms and molecular basis of the rumpless trait in Piao chicken. Through genome-wide association and linkage analyses, the candidate region was fine-mapped to 798.5 kb (chromosome 2: 86.9 to 87.7 Mb). Whole-genome sequencing analyses identified a single variant, a 4.2 kb deletion, which was completely associated with the rumpless phenotype. Explorations of the expression data identified a novel causative gene, Rum, that produced a long, intronless transcript across the deletion. The expression of Rum is embryo-specific, and it regulates the expression of MSGN1, a key factor in regulating T-box transcription factors required for mesoderm formation and differentiation. These results provide genetic and molecular experimental evidence for a novel mechanism regulating tail development in chicken and report the likely causal mutation for the tail abnormity in the Piao chicken. The novel regulatory gene, Rum, will, due to its role in fundamental embryo development, be of interest for further explorations of a potential role in tail and skeletal development also in other vertebrates.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Genetik och förädling inom lantbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Genetics and Breeding in Agricultural Sciences (hsv//eng)
Nyckelord
- rumpless
- Piao chicken
- genetic mapping
- functional analyses
- somitogenesis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Guo, Ying
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Tian, Jing
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Song, Chi
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Han, Wei
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Zhu, Chunhong
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Li, Huifang
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visa fler...
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Zhang, Shuangjie
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Chen, Kuanwei
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Li, Ning
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Carlborg, Örjan
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Hu, Xiaoxiang
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Genetik
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Molecular biolog ...
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Uppsala universitet