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ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat
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- Nishio, Shunsuke (author)
- Karolinska Inst, Dept Biosci & Nutr, Huddinge, Sweden.;Fukushima Univ, Inst Fermentat Sci IFeS, Fac Food & Agr Sci, Fukushima, Japan.
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- Emori, Chihiro (author)
- Osaka Univ, Res Inst Microbial Dis, Dept Expt Genome Res, Suita, Osaka, Japan.;Osaka Univ, Immunol Frontier Res Ctr, Suita, Osaka, Japan.
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- Wiseman, Benjamin (author)
- Karolinska Inst, Dept Biosci & Nutr, Huddinge, Sweden.;Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.
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- Karolinska Inst, Dept Biosci & Nutr, Huddinge, Sweden;Fukushima Univ, Inst Fermentat Sci IFeS, Fac Food & Agr Sci, Fukushima, Japan. Osaka Univ, Res Inst Microbial Dis, Dept Expt Genome Res, Suita, Osaka, Japan.;Osaka Univ, Immunol Frontier Res Ctr, Suita, Osaka, Japan. (creator_code:org_t)
- Elsevier, 2024
- 2024
- English.
- In: Cell. - : Elsevier. - 0092-8674 .- 1097-4172. ; 187:6, s. 1440-1459.e24
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Following the fertilization of an egg by a single sperm, the egg coat or zona pellucida (ZP) hardens and polyspermy is irreversibly blocked. These events are associated with the cleavage of the N -terminal region (NTR) of glycoprotein ZP2, a major subunit of ZP filaments. ZP2 processing is thought to inactivate sperm binding to the ZP, but its molecular consequences and connection with ZP hardening are unknown. Biochemical and structural studies show that cleavage of ZP2 triggers its oligomerization. Moreover, the structure of a native vertebrate egg coat filament, combined with AlphaFold predictions of human ZP polymers, reveals that two protofilaments consisting of type I (ZP3) and type II (ZP1/ZP2/ZP4) components interlock into a left-handed double helix from which the NTRs of type II subunits protrude. Together, these data suggest that oligomerization of cleaved ZP2 NTRs extensively cross -links ZP filaments, rigidifying the egg coat and making it physically impenetrable to sperm.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- fertilization
- egg-sperm interaction
- block to polyspermy
- egg coat hardening
- zona pellucida
- ZP2 cleavage
- ZP1
- ZP3
- ZP protein filament
- integrative structural biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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