Sökning: id:"swepub:oai:DiVA.org:uu-63677" >
Expression of hyalu...
Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors.
-
- Jacobson, Annica (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Rahmanian, Mehdi (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Rubin, Kristofer (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
visa fler...
-
- Heldin, Paraskevi (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Ludwiginstitutet för Cancerforskning
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2002
- 2002
- Engelska.
-
Ingår i: Int J Cancer. - : Wiley. ; 102:3, s. 212-9
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Advanced colorectal cancers are often associated with elevated amounts of hyaluronan. To investigate the importance of hyaluronan in colon carcinoma tumor progression, we have expressed by stable transfection hyaluronan synthase 2 (Has2) and hyaluronidase 1 (Hyal1) in the rat colon carcinoma cell line, PROb. We found that hyaluronan overproduction led to a higher growth rate of tumor cells in vitro, and to a faster development of transplantable tumors in syngeneic rats, compared to the mock-transfectants. Has2 transfected PROb cells gave rise to tumors that were significantly less vascularized, but had a significantly larger viable tumor fraction compared to tumors generated from mock-transfectants. In contrast, Hyal1 overexpression suppressed the growth rate of tumor cells both in vitro and in vivo. Moreover, tumors derived from Hyal1-transfected cells had a significantly larger necrotic area than tumors derived from mock- and Has2-transfectants. Our study demonstrates that Has2 overproduction promotes tumorigenicity, whereas Hyal1 overexpression suppresses tumorigenicity in an experimental model for colon carcinoma.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas